|
| Status |
Public on Feb 09, 2008 |
| Title |
GFP-progerin Induction: day 5 Replicate B (5b_progerin_5d_B) |
| Sample type |
RNA |
| |
|
| Source name |
hTERT-immortalized human skin fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
Cell line expressing inducible GFP-progerin. Induction: day 5. Replicate B
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted with Qiagen coulumns
|
| Label |
Biotin
|
| Label protocol |
First and second strand cDNA was prepared from the total RNA samples
Biotinylated cRNA target was prepared from the DNA template.
|
| |
|
| Hybridization protocol |
cRNA was fragmented to uniform size.
CodeLink Bioarrays were hybridized with the cRNA target and stained with Cy5-streptavidin
|
| Scan protocol |
Slides were washed and then scanned on an Axon GenePix 4000B scanner
|
| Description |
Labelling, hybridisation and data analysis was performed by GenUs Biosystems
|
| Data processing |
Data was analyzed with CodeLink and GeneSpring software packages
|
| |
|
| Submission date |
Jan 09, 2008 |
| Last update date |
Jan 10, 2008 |
| Contact name |
paola scaffidi |
| E-mail(s) |
scaffidp@mail.nih.gov
|
| Organization name |
NCI, NIH
|
| Street address |
41 Library Drive, B508
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE10123 |
Progerin-induced changes in gene expression |
|
