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| Status |
Public on Feb 09, 2008 |
| Title |
Progerin-induced changes in gene expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The premature aging disease Hutchinson-Gilford Progeria Syndrome (HGPS) is caused by constitutive production of progerin, a mutant form of the nuclear architectural protein lamin A1. Progerin is also sporadically expressed in wild type cells and has been linked to physiological aging. HGPS cells exhibit extensive nuclear defects including abnormal chromatin structure and increased DNA damage. At the organismal level, HGPS affects several tissues particularly of mesenchymal origin. How the cellular defects of HGPS cells lead to the organismal defects has been unclear. To begin to unravel how progerin leads to disease phenotypes, we analyzed time-dependent changes in transcriptional profiles in response to progerin expression in a hTERT-immortalized skin fibroblast cell line expressing either GFP-progerin or the GFP-wt lamin A control
Keywords: time course, cell line comparison
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| Overall design |
Three time points (0d, 5d, 10d after induction of the exogenous protein) were analyzed for each cell line (expressing either GFP-progerin or GFP-wt lamin A). Two biological replicates were analyzed for each time point.
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| Contributor(s) |
Scaffid P, Misteli T |
| Citation(s) |
18311132 |
| Submission date |
Jan 09, 2008 |
| Last update date |
Oct 28, 2014 |
| Contact name |
paola scaffidi |
| E-mail(s) |
scaffidp@mail.nih.gov
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| Organization name |
NCI, NIH
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| Street address |
41 Library Drive, B508
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL2895 |
GE Healthcare/Amersham Biosciences CodeLink Human Whole Genome Bioarray |
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| Samples (12)
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| GSM255774 |
WT-GFP-lamin A Induction: Day 0 Replicate A (CBGE_Hsapiens_WG_progerin1) |
| GSM255806 |
WT-GFP-lamin A Induction: day 0 Replicate B (1b_WT_0d_B) |
| GSM255807 |
WT-GFP-lamin A Induction: day 5 Replicate A (2a_WT_5d_A) |
| GSM255808 |
WT-GFP-lamin A Induction: day 5 Replicate B (2b_WT_5d_B) |
| GSM255809 |
WT-GFP-lamin A Induction: day 10 Replicate A (3a_WT_10d_A) |
| GSM255828 |
WT-GFP-lamin A Induction: day 10 Replicate B (3b_WT_10d_B) |
| GSM255829 |
GFP-progerin Induction: day 0 Replicate A (4a_progerin_0d_A) |
| GSM255830 |
GFP-progerin Induction: day 0 Replicate B (4b_progerin_0d_B) |
| GSM255831 |
GFP-progerin Induction: day 5 Replicate A (5a_progerin_5d_A) |
| GSM255832 |
GFP-progerin Induction: day 5 Replicate B (5b_progerin_5d_B) |
| GSM255833 |
GFP-progerin Induction: day 10 Replicate A (6a_progerin_10d_A) |
| GSM255834 |
GFP-progerin Induction: day 10 Replicate B (6b_progerin_10d_B) |
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| Relations |
| BioProject |
PRJNA108667 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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