|
| Status |
Public on Jun 23, 2020 |
| Title |
wt-MCF7 991R |
| Sample type |
genomic |
| |
|
| Source name |
MCF7 in E2 treated with Palbociclib (CDK4/6 inhibitor) until resistant
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: wt-MCF7 991R
cell line: MCF7
compound: E2 treated with Palbociclib (CDK4/6 inhibitor) until resistant
|
| Treatment protocol |
wt cells were treated long term with palobociclib until thay develop resistance.
|
| Growth protocol |
Cell lines were grown according to standard conditions.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from target cell lines using QIAamp DNA mini kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, PicoGreen and agarose gel electrophoresis.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the GeneChip® Scanner 3000 7G with AutoLoader.
|
| Description |
Palbociclib-resistant
|
| Data processing |
SNPs were called using the Birdseed v2 algorithm (.CHP files), and copy number variations were called using the CN5 algorithm (.CNCHP files) using Affymetrix Genotyping Console 4.0 (Affymetrix). Sample table and .CNCHP data are based on genome build GRCh37 (hg19). HapMap samples were used as a reference.
Genotype Call (SNP call): AA, AB, BB, and NoCall; 'Signal' = Summarized signal
|
| |
|
| Submission date |
May 17, 2017 |
| Last update date |
Jun 23, 2020 |
| Contact name |
Alice Gao |
| E-mail(s) |
alice.gao@icr.ac.uk
|
| Organization name |
The Institute of Cancer Research
|
| Department |
Breast Cancer Now
|
| Lab |
Molecular Cell Biology
|
| Street address |
237 Fulham Road
|
| City |
London |
| State/province |
London |
| ZIP/Postal code |
SW3 6JB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6801 |
| Series (2) |
| GSE99002 |
Resistance to Palbociclib involves multiple mechanisms amenable to targeting with drug holidays or drug switching to improve therapeutic outcome [Affymetrix] |
| GSE99003 |
Resistance to Palbociclib involves multiple mechanisms amenable to targeting with drug holidays or drug switching to improve therapeutic outcome |
|
