|
| Status |
Public on Jun 30, 2018 |
| Title |
pancreatic cancer cell, non-irradiated, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
pancreatic cancer cell line, non-irradiated
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: pancreatic cancer
cell line: PANC-1
treatment: non-irradiated
|
| Treatment protocol |
Pancreatic cancer cells were irradiated with 10Gy/0Gy using an Oncor linear accelerator, and collected at 24h after radiation for RNA extraction and hybridization on Affymetrix microarray.
|
| Growth protocol |
Pancreatic cancer cells were seeded into culture plate overnight with 2% FBS in culture medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated ds cDNA were prepared according to the standard Affymetrix protocol from 6.25 ug fragmented ds Cdna
|
| |
|
| Hybridization protocol |
Following labelling, ~20 ug of cDNA were hybridized at 45C hybridization oven, at 60 rpm for 16 hr on Array. GeneChips were washed and stained with Affymetrix® Hybridization, Wash and Stain Kit using the FS450_0001 fluidics protocol
|
| Scan protocol |
Arrays were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
whole transcript data from PANC-1 cells without irradiation
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix Expression Console™ Software Version 1.0. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 13, 2017 |
| Last update date |
Jun 30, 2018 |
| Contact name |
Ling Tian |
| E-mail(s) |
TL09168@hotmail.com
|
| Organization name |
Shanghai General Hospital
|
| Department |
Institute of Translational Medicine
|
| Lab |
Shanghai Key Laboratory of Pancreatic Diseases
|
| Street address |
No. 650, New Songjiang Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
201620 |
| Country |
China |
| |
|
| Platform ID |
GPL16238 |
| Series (1) |
| GSE104965 |
The effects of X-ray radiation on pancreatic cancer cells |
|
