|
| Status |
Public on Jul 30, 2008 |
| Title |
Neutrophils cultured from wild-type mouse bone marrow - Rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
neutrophils
|
| Organism |
Mus musculus |
| Characteristics |
mRNA profiling of neutrophils in vitro cultured from wild-type mouse bone marrow - Rep 1
|
| Treatment protocol |
Live neutrophils were isolated using MACS.
|
| Growth protocol |
BM-progenitors were cultured in SILAC-IMDM with G-CSF and SCF for 6 days and subsequent culture for 42 hours without SCF. During the time, maturation to neutrophils occurs.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tri Reagent (Ambion) followed by RNeasy Plus (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Affy Standard labeling Protocol
|
| |
|
| Hybridization protocol |
Affy Standard Hyb Protocol
|
| Scan protocol |
Affy Scanner
|
| Description |
mRNA expression profile comparison between wild-type cultured murine neutrophils versus miR-223 null cultured murine neutrophils
|
| Data processing |
GCOS and RMAExpress software
|
| |
|
| Submission date |
Jul 02, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Whitehead Institute |
| E-mail(s) |
sgupta@wi.mit.edu
|
| Phone |
617-324-0339
|
| Organization name |
Whitehead Institute
|
| Department |
Genome Technology Core
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02141 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE11973 |
Wild-type cultured neutrophils versus miR-223 null cultured neutrophils |
| GSE12075 |
The impact of microRNAs on protein output |
|
| Relations |
| Reanalyzed by |
GSE45704 |
| Reanalyzed by |
GSE119085 |
