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Sample GSM303276 Query DataSets for GSM303276
Status Public on Jul 30, 2008
Title Neutrophils cultured from wild-type mouse bone marrow - Rep 3
Sample type RNA
Source name Neutrophils
Organism Mus musculus
Characteristics mRNA profiling of neutrophils in vitro cultured from wild-type mouse bone marrow - Rep 3
Treatment protocol Live neutrophils were isolated using MACS.
Growth protocol BM-progenitors were cultured in SILAC-IMDM with G-CSF and SCF for 6 days and subsequent culture for 42 hours without SCF. During the time, maturation to neutrophils occurs.
Extracted molecule total RNA
Extraction protocol Tri Reagent (Ambion) followed by RNeasy Plus (Qiagen)
Label Biotin
Label protocol Affy Standard labeling Protocol
Hybridization protocol Affy Standard Hyb Protocol
Scan protocol Affy Scanner
Description mRNA expression profile comparison between wild-type cultured murine neutrophils versus miR-223 null cultured murine neutrophils
Data processing GCOS and RMAExpress software
Submission date Jul 02, 2008
Last update date Aug 28, 2018
Contact name Whitehead Institute
Phone 617-324-0339
Organization name Whitehead Institute
Department Genome Technology Core
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
Platform ID GPL1261
Series (2)
GSE11973 Wild-type cultured neutrophils versus miR-223 null cultured neutrophils
GSE12075 The impact of microRNAs on protein output
Reanalyzed by GSE45704
Reanalyzed by GSE119085

Data table header descriptions
VALUE Signal Intensity (Log2)

Data table
1415670_AT 9.223097
1415671_AT 11.191702
1415672_AT 11.207906
1415673_AT 8.765438
1415674_A_AT 9.698643
1415675_AT 9.010616
1415676_A_AT 11.730345
1415677_AT 9.973807
1415678_AT 10.381256
1415679_AT 11.846189
1415680_AT 9.395477
1415681_AT 9.855852
1415682_AT 7.801260
1415683_AT 11.097977
1415684_AT 9.335694
1415685_AT 8.694534
1415686_AT 9.562251
1415687_A_AT 13.171519
1415688_AT 9.724204
1415689_S_AT 7.798752

Total number of rows: 45101

Table truncated, full table size 902 Kbytes.

Supplementary file Size Download File type/resource
GSM303276.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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