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Sample GSM324677 Query DataSets for GSM324677
Status Public on Dec 01, 2008
Title Patient_#2000_C3
Sample type protein
 
Source name serum
Organism Homo sapiens
Characteristics anti-dsDNA:positive
SEX: Male
Growth protocol Serum samples were stored at -70C
Extracted molecule protein
Extraction protocol Serum sample was centrifuged for 6min at 15000g for removing particles
Label Cy5
Label protocol Labeling with fluorescent antibodies was carried out at room temperature for 30 min in 5% BSA and 0.05% Tween 20 containing PBS.
 
Hybridization protocol Dried arrays were rinsed in PBS for 15 minutes before use, then incubated with 5 times diluted serum at 37°C for 1 hour. Sera were diluted in 5% BSA, 0.05% Tween 20 Ca and Mg supplemented Veronal buffer. Serum treated slides were washed with 0.05% Tween 20 containing PBS, then incubated in 1:5000 diluted Cy5-conjugated F(ab')2 fragment goat anti-human IgM (Jackson ImmunoResearch). Following washing in 0.05% Tween 20 containing PBS, arrays were dried and scanned.
Scan protocol Arrays were scanned on Typhoon Trio+ imager (Amersham Bioscience) (Excitation:633nm ; Emission: 670 BP30 , PMT:410 Mode:Fluorescence, Sensitivity:Normal, FocalPlane=+3 mm). Data were analyzed with ImageQuantTL (Amersham Bioscience) software.
Description C3-2000
Data processing Background derived from marked spots, was substracted from signal. For interassay comparison, data were normalized to the mean of two dilutions of printed anti-human IgG+IgM control material (ID_REF: #539, #571, #603, #355, #387, #419). Only data derived from slides with normalization factor between 0.4 and 2.5 were considered suitable for analysis. Based on the distribution of data normalized fluorescence intensity signals smaller than 1000 for IgM were rounded up to these values.
 
Submission date Sep 26, 2008
Last update date Nov 13, 2008
Contact name József Prechl
E-mail(s) jprechl@iif.hu
Phone +3613812175
Fax +3613812176
URL http://www.abc-arrays.com
Organization name ELTE-MTA
Department Immunology Research Group
Lab Immunoproteomics
Street address Pázmány Péter s. 1/C
City Budapest
ZIP/Postal code 1117
Country Hungary
 
Platform ID GPL7390
Series (1)
GSE12943 Profiling antibody function on antigen arrays

Data table header descriptions
ID_REF
VALUE normalized fluorescence intensity signal

Data table
ID_REF VALUE
1 33472.66818
2 575874.531
3 575389.9219
4 1686783.208
5 292744.4733
6 118441.813
7 490153.9263
8 74965.68992
9 92134.81765
10 120241.2157
11 89807.00994
12 444844.2587
13 87395.81499
14 26094.75294
15 42088.73128
16 1000
17 1000
18 1000
19 21323.241
20 172735.6807

Total number of rows: 960

Table truncated, full table size 12 Kbytes.




Supplementary file Size Download File type/resource
GSM324677.txt.gz 9.0 Kb (ftp)(http) TXT
Processed data included within Sample table

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