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Sample GSM3635616 Query DataSets for GSM3635616
Status Public on Apr 27, 2020
Title miR-138_293T2
Sample type SRA
Source name 293T cells
Organism Homo sapiens
Characteristics tissue: Kidney
cell line: 293T
transfected with: miR-138 mimic
Treatment protocol Neuro-2a cells were transfected with 40 nM of a miR-138 or scrambled mimic for 24 h before performing RNAseq
Extracted molecule total RNA
Extraction protocol Total RNAs were purified using an RNeasyPlus Mini Kit following the manufacturer’s instructions (Qiagen)
mRNAs were isolated using a Takara mRNA Isolation Kit. Libraries were prepared using a PrepX RNA-seq Library Preparation Kit
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Description miR-138 transfected 293T cells
Data processing Reads were trimmed to remove adapters, low-quality reads, and reads less than 30 bases long using Trimmomatic (
The trimmed reads were aligned with the human (hg19 build) or mouse (mm10 build) using Tophat2 (
Counts of reads aligned to each transcript were determined by Htseq-count (
Genome_build: hg19 (human), mm10 (mouse)
Supplementary_files_format_and_content: Tab-delimited text files containing raw counts of sequencing reads for each viral gene
Submission date Feb 28, 2019
Last update date Apr 28, 2020
Contact name Dongli Pan
Organization name Zhejiang University
Street address 866 Yuhangtang Road
City Hangzhou
State/province Zhejiang
ZIP/Postal code 110058
Country China
Platform ID GPL18573
Series (2)
GSE127502 Identification of targets of miR-138 in Neuro-2a and 293T cells by RNAseq
GSE127504 Identification of herpes simplex virus 1 and host targets of miR-138 by combined RNAseq/PARCLIP
BioSample SAMN11037847
SRA SRX5444374

Supplementary file Size Download File type/resource
GSM3635616_Ehuman_counts.txt.gz 102.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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