|
| Status |
Public on Apr 10, 2019 |
| Title |
C2.I5.22_X_C2.I5.pool |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
3280.C2.I5.22
|
| Organism |
Saccharum hybrid cultivar SP80-3280 |
| Characteristics |
tissue: Internode 5
variety: SP80-3280
collect: 22/01/2013
|
| Treatment protocol |
NO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
modified Trizol method
|
| Label |
Cy3
|
| Label protocol |
CyScribe Post-Labelling Kit Protocol - GE Healthcare
|
| |
|
| Channel 2 |
| Source name |
3280.C2.I5.pool
|
| Organism |
Saccharum hybrid cultivar SP80-3280 |
| Characteristics |
tissue: Internode 5
variety: SP80-3280
collect: 22/01/2013
|
| Treatment protocol |
NO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
modified Trizol method
|
| Label |
Cy5
|
| Label protocol |
CyScribe Post-Labelling Kit Protocol - GE Healthcare
|
| |
|
| |
| Hybridization protocol |
Two-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling) Protocol
|
| Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000B Scanner using Agilent Settings and image intensity data were extracted with Feature Extraction Software using Agilent Settings
|
| Description |
Comparison between RNA Internode 5 C2C522 time point sample and RNA pool sample.
|
| Data processing |
Raw and background corrected data
|
| |
|
| Submission date |
Apr 09, 2019 |
| Last update date |
Apr 10, 2019 |
| Contact name |
Carlos T Hotta |
| E-mail(s) |
hotta@iq.usp.br
|
| Organization name |
University of Sao Paulo
|
| Department |
Biochemistry
|
| Lab |
Plant Molecular Physiology
|
| Street address |
Av. Lineu Prestes, 748
|
| City |
Sao Paulo |
| State/province |
SP |
| ZIP/Postal code |
05508010 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL14862 |
| Series (1) |
| GSE129543 |
Vast differences in organ-specific rhythms of transcription in field-grown sugarcane |
|
| Data table header descriptions |
| ID_REF |
ID column of the reference platform |
| VALUE |
log2(gProcessedSignal/rProcessedSignal) |
| gSignal |
Raw Median Signal of feature in green channel |
| rSignal |
Raw Median Signal of feature in red channel |
| gProcessedSignal |
Dye-normalized signal after surrogate "algorithm,"green "channel,"used for computation of log ratio. |
| rProcessedSignal |
Dye-normalized signal after surrogate "algorithm,"red "channel,"used for computation of log ratio. |
