|
Status |
Public on Apr 10, 2019 |
Title |
C2.F1.pool_X_C2.F1.04 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
3280.C2.F1.04
|
Organism |
Saccharum hybrid cultivar SP80-3280 |
Characteristics |
tissue: Leaf 1 variety: SP80-3280 collect: 22/01/2013
|
Treatment protocol |
NO
|
Extracted molecule |
total RNA |
Extraction protocol |
modified Trizol method
|
Label |
Cy5
|
Label protocol |
CyScribe Post-Labelling Kit Protocol - GE Healthcare
|
|
|
Channel 2 |
Source name |
3280.F1.C2.pool
|
Organism |
Saccharum hybrid cultivar SP80-3280 |
Characteristics |
tissue: Leaf 1 variety: SP80-3280 collect: 22/01/2013
|
Treatment protocol |
NO
|
Extracted molecule |
total RNA |
Extraction protocol |
modified Trizol method
|
Label |
Cy3
|
Label protocol |
CyScribe Post-Labelling Kit Protocol - GE Healthcare
|
|
|
|
Hybridization protocol |
Two-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling) Protocol
|
Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000B Scanner using Agilent Settings and image intensity data were extracted with Feature Extraction Software using Agilent Settings
|
Description |
Swap of comparison between Leaf 1 pool sample and C2F104 time point sample.
|
Data processing |
Raw and background corrected data
|
|
|
Submission date |
Apr 09, 2019 |
Last update date |
Apr 10, 2019 |
Contact name |
Carlos T Hotta |
E-mail(s) |
hotta@iq.usp.br
|
Organization name |
University of Sao Paulo
|
Department |
Biochemistry
|
Lab |
Plant Molecular Physiology
|
Street address |
Av. Lineu Prestes, 748
|
City |
Sao Paulo |
State/province |
SP |
ZIP/Postal code |
05508010 |
Country |
Brazil |
|
|
Platform ID |
GPL14862 |
Series (1) |
GSE129543 |
Vast differences in organ-specific rhythms of transcription in field-grown sugarcane |
|
Data table header descriptions |
ID_REF |
ID column of the reference platform |
VALUE |
log2(rProcessedSignal/gProcessedSignal) |
gSignal |
Raw Median Signal of feature in green channel |
rSignal |
Raw Median Signal of feature in red channel |
gProcessedSignal |
Dye-normalized signal after surrogate "algorithm,"green "channel,"used for computation of log ratio. |
rProcessedSignal |
Dye-normalized signal after surrogate "algorithm,"red "channel,"used for computation of log ratio. |