Total RNA was prepared using RNAzol B reagent (Tel-Test, Friendswood, TX) from five each independent cultures of AGS and AGS-EBV cells. The quantity and quality of total RNA were assessed with a Bioanalyzer (Agilent Technologies, Santa Clara, CA) and Experion (Bio-Rad, Hercules, CA).
Label
biotin
Label protocol
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol
Standard Illumina hybridization protocol
Scan protocol
Microarrays were scanned using an Illumina BeadArray Reader.
Description
AGS independent culture 4 N4
Data processing
statistical analyses were performed using Geneplex 2.3 software (ISTECH Inc., Goyang, Korea).
