|
Status |
Public on Feb 12, 2010 |
Title |
UTX input_Lung_1of2 |
Sample type |
genomic |
|
|
Source name |
Input
|
Organism |
Homo sapiens |
Characteristics |
cell type: lung primary human fibroblasts
|
Growth protocol |
Fibroblasts were grown in DMEM with 10% Fetal Bovine Serum until ~70% confluent
|
Extracted molecule |
genomic DNA |
Extraction protocol |
ChIP was performed on 4x10^6 fibroblast cells and Farnham ChIP protocol was used to derive ChIP DNA and was amplified by sigma WGA2 kit for 1 round of amplification
|
Label |
Cy3
|
Label protocol |
Standard Nimblegen ChIP labeling protocol (1 ug of Amplified ChIP DNA, direct incorporation of cy labeled primers with klenow extension
|
|
|
Hybridization protocol |
Standard Nimblegen hybridization kits and protocols were used
|
Scan protocol |
Arrays were scanned with Axon scanner at 5 um resolution according to standard Nimblegen protocols
|
Description |
none
|
Data processing |
Arrays were processed using Nimblegens standard protocol for Nimblescan 2.4 ChIP data extraction
|
|
|
Submission date |
May 26, 2009 |
Last update date |
Feb 12, 2010 |
Contact name |
Jordon K Wang |
E-mail(s) |
jordon@stanford.edu
|
Phone |
650-724-9140
|
Fax |
650-723-8762
|
Organization name |
Stanford University
|
Department |
Dermatology
|
Lab |
CCSR 4124
|
Street address |
269 Campus Dr.
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
|
|
Platform ID |
GPL6325 |
Series (1) |
GSE16221 |
UTX Chromatin Immunoprecipitation on human HG18 Nimblegen 2 array promoter set |
|