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Sample GSM407350 Query DataSets for GSM407350
Status Public on Feb 12, 2010
Title UTX input_Lung_1of2
Sample type genomic
 
Source name Input
Organism Homo sapiens
Characteristics cell type: lung primary human fibroblasts
Growth protocol Fibroblasts were grown in DMEM with 10% Fetal Bovine Serum until ~70% confluent
Extracted molecule genomic DNA
Extraction protocol ChIP was performed on 4x10^6 fibroblast cells and Farnham ChIP protocol was used to derive ChIP DNA and was amplified by sigma WGA2 kit for 1 round of amplification
Label Cy3
Label protocol Standard Nimblegen ChIP labeling protocol (1 ug of Amplified ChIP DNA, direct incorporation of cy labeled primers with klenow extension
 
Hybridization protocol Standard Nimblegen hybridization kits and protocols were used
Scan protocol Arrays were scanned with Axon scanner at 5 um resolution according to standard Nimblegen protocols
Description none
Data processing Arrays were processed using Nimblegens standard protocol for Nimblescan 2.4 ChIP data extraction
 
Submission date May 26, 2009
Last update date Feb 12, 2010
Contact name Jordon K Wang
E-mail(s) jordon@stanford.edu
Phone 650-724-9140
Fax 650-723-8762
Organization name Stanford University
Department Dermatology
Lab CCSR 4124
Street address 269 Campus Dr.
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL6325
Series (1)
GSE16221 UTX Chromatin Immunoprecipitation on human HG18 Nimblegen 2 array promoter set

Data table header descriptions
ID_REF
VALUE SIGNAL_MEAN
SIGNAL_STDEV

Data table
ID_REF VALUE SIGNAL_STDEV
CHR07P091999937 1962.44 485.15
CHR04P006203286 10071.11 931.70
CHR03P048686553 3978.78 887.83
CHR01P054643545 1016.78 105.49
CHR08P038245065 1671.11 399.91
CHR07P006018514 11544.78 1269.23
CHR04P001049036 7379.67 1530.98
CHR06P070632668 3204.33 733.93
CHR08P144708434 1249.78 403.45
CHR01P154098538 3055.78 924.27
CHR09P033784358 12141.78 3198.20
CHR03P039120694 2684.44 334.83
CHR06P043637870 2318.56 451.66
CHR03P043088083 2620.22 696.94
CHR06P151307434 718.89 132.87
CHR09P138560709 1650.33 351.65
CHR03P124652734 872.89 185.38
CHR04P025476441 4047.78 460.02
CHR06P138229950 2810.22 604.43
CHR09P004656327 4241.33 751.82

Total number of rows: 378943

Table truncated, full table size 11449 Kbytes.




Supplementary data files not provided
Processed data not provided for this record

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