|
| Status |
Public on May 21, 2010 |
| Title |
yeast treated with CQ, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
yeast treated with 80 µM CQ for 5 hours
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BY4742
media supplement: 80 µM CQ
|
| Treatment protocol |
One percent (v/v) DMSO (D) or 80 µM CQ (Q) was supplemented into the yeast culture medium.
|
| Growth protocol |
Wild type BY4742 yeast strain grown in YPD to mid-log phase were inoculated 1:1 into fresh YPD supplemented with either DMSO (D) or CQ (Q) and grown at 30℃ at 175 rpm in a shaking incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the hot phenol method (Collart, M. A., and Oliviero, S. (2001) Curr Protoc Mol Biol Chapter 13, Unit13 12).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization was performed according to standard Affymetrix procedures. Briefly, 10ug cRNA was hybridized for 16hr at 45℃ on Affymetrix GeneChip® Yeast Genome 2.0 Arrays in an Affymetrix ® Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix ® GeneChip® Scanner 3000.
|
| Description |
080821-Yeast-08110B_1Qa
|
| Data processing |
Normalization and expression analysis was performed using DNA-chip analyzer (dChip). Invariant Set Normalization was used to normalize arrays at the probe level, and the model-based method was used to calculate expression values.
|
| |
|
| Submission date |
Jul 22, 2009 |
| Last update date |
May 21, 2010 |
| Contact name |
Chenghua Li |
| E-mail(s) |
ch-li06@mails.thu.edu.cn
|
| Organization name |
Tsinghua University
|
| Department |
Biological Sciences and Technology
|
| Street address |
Qinghuayuan
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE17257 |
Expression data of Saccharomyces cerevisiae treated with clioquinol |
|
