|
| Status |
Public on Jan 27, 2021 |
| Title |
BALBc p18null BRCA1het 316 XIONG |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mammary tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balbc
tissue: mammary tumor
genotype: p18null_BRCA1het
|
| Treatment protocol |
NA
|
| Growth protocol |
Mammary tumors were harvested for gene expression analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen RNeasy kits.
|
| Label |
Cy5
|
| Label protocol |
Described in Hu et al., 2005, BioTechniques, 38:121-125.
|
| |
|
| Channel 2 |
| Source name |
Normal reference
|
| Organism |
Mus musculus |
| Characteristics |
sample type: reference
genotype: normal
|
| Treatment protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Reference
|
| Label |
Cy3
|
| Label protocol |
Described in Hu et al., 2005, BioTechniques, 38:121-125.
|
| |
|
| |
| Hybridization protocol |
Described in Hu et al., 2005, BioTechniques, 38:121-125.
|
| Scan protocol |
Described in Hu et al., 2005, BioTechniques, 38:121-125.
|
| Description |
Agilent-850
mRNA isolated from flash frozen bulk tumors.
|
| Data processing |
Data for both channels were Lowess-normalized and then the log(2) ratio was taken. Both channels were filtered for Lowess Normalized Net (Mean) >= 10.
|
| |
|
| Submission date |
Aug 07, 2020 |
| Last update date |
Jan 27, 2021 |
| Contact name |
Charles M. Perou |
| E-mail(s) |
cperou@med.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Professor of Genetics, and Pathology & Laboratory Medicine; Lineberger Comprehensive Cancer Center
|
| Street address |
12-044 Lineberger Comprehensive Cancer Center CB# 7295
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7264 |
| Country |
USA |
| |
|
| Platform ID |
GPL10732 |
| Series (1) |
| GSE155239 |
Understanding BRCA1 function in INK4-RB deficient tumors |
|
