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Sample GSM5493608 Query DataSets for GSM5493608
Status Public on Sep 02, 2021
Title 138 DC
Sample type RNA
 
Source name TRUC mouse
Organism Mus musculus
Characteristics tissue: Mouse distal colons
treatment group: isotype control
Extracted molecule total RNA
Extraction protocol distal colons were preserved snap frozen in RNAlater, homogenized in 1 mL of Trizol Reagent (Invitrogen cat. #15596-026) with a 5 mm stainless steel bead (Qiagen cat. #69989) in RNase-free tubes (Ambion) on a tissue homogenizer (Tissue Lyser II) for 3 runs X 2 minutes at frequency 25.0 for 5 minutes at room temperature to permit complete dissociation. 200 μL of chloroform (EM Science cat. # CX1055-6) per mL of Trizol Reagent was added, capped securely and shaken vigorously by hand for 15 seconds. Tubes were incubated at room temperature for 15 minutes and then centrifuged at 10,000 x g for 10 min. at 4 degrees C. The top aqueous phase was harvested after centrifugation and transferred to 96 well deep well plates and stored at -80 degrees C until RNA isolation. RNA was isolated with automation on the Biomek using Agencourt RNAdvance Tissue Kit (Beckman Coulter #14073200). Yield and quality of RNA were determined using Nanodrop and RNA Integrity Number (RIN) values were determined using Tapestation (Agilent)
Label n/a
Label protocol n/a
 
Hybridization protocol n/a
Scan protocol n/a
Data processing All samples were normalized to the same concentration and a total of 100 ng of RNA per sample were used for hybridization and run on the XT_PGX_MmV2_Inflammation_CSO 248 gene panel (NanoString Item No. 115000082)
 
Submission date Jul 31, 2021
Last update date Sep 02, 2021
Contact name Li Li
E-mail(s) frank.li@boehringer-ingelheim.com
Organization name Boehringer Ingelheim Pharmaceuticals, Inc.
Street address PO Box 368
City Ridgefield
State/province Connecticut
ZIP/Postal code 06877-0368
Country USA
 
Platform ID GPL20885
Series (1)
GSE181253 The potent and selective RIPK2 inhibitor BI 706039 improves intestinal inflammation in the TRUC mouse model of Inflammatory Bowel Disease

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
Ager 6.148252656
Alox12 -7.658349799
Alox15 70.00378901
Alox5 19.09194246
Areg 957.9409094
Arg1 283.1432144
Atf2 1112.402274
Bcl2l1 421.209239
Bcl6 36.35019553
Birc2 597.2434203
C1qa 2167.74445
C1qb 1931.306382
C1ra 291.7723409
C1s 3069.488172
C2 347.8616634
C3 2295.455522
C3ar1 381.5152569
C4a 2020.186386
C6 230.5055426
C7 44.97932206

Total number of rows: 248

Table truncated, full table size 4 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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