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Sample GSM5493620 Query DataSets for GSM5493620
Status Public on Sep 02, 2021
Title 173 DC
Sample type RNA
 
Source name TRUC mouse
Organism Mus musculus
Characteristics tissue: Mouse distal colons
treatment group: anti-p40
Extracted molecule total RNA
Extraction protocol distal colons were preserved snap frozen in RNAlater, homogenized in 1 mL of Trizol Reagent (Invitrogen cat. #15596-026) with a 5 mm stainless steel bead (Qiagen cat. #69989) in RNase-free tubes (Ambion) on a tissue homogenizer (Tissue Lyser II) for 3 runs X 2 minutes at frequency 25.0 for 5 minutes at room temperature to permit complete dissociation. 200 μL of chloroform (EM Science cat. # CX1055-6) per mL of Trizol Reagent was added, capped securely and shaken vigorously by hand for 15 seconds. Tubes were incubated at room temperature for 15 minutes and then centrifuged at 10,000 x g for 10 min. at 4 degrees C. The top aqueous phase was harvested after centrifugation and transferred to 96 well deep well plates and stored at -80 degrees C until RNA isolation. RNA was isolated with automation on the Biomek using Agencourt RNAdvance Tissue Kit (Beckman Coulter #14073200). Yield and quality of RNA were determined using Nanodrop and RNA Integrity Number (RIN) values were determined using Tapestation (Agilent)
Label n/a
Label protocol n/a
 
Hybridization protocol n/a
Scan protocol n/a
Data processing All samples were normalized to the same concentration and a total of 100 ng of RNA per sample were used for hybridization and run on the XT_PGX_MmV2_Inflammation_CSO 248 gene panel (NanoString Item No. 115000082)
 
Submission date Jul 31, 2021
Last update date Sep 02, 2021
Contact name Li Li
E-mail(s) frank.li@boehringer-ingelheim.com
Organization name Boehringer Ingelheim Pharmaceuticals, Inc.
Street address PO Box 368
City Ridgefield
State/province Connecticut
ZIP/Postal code 06877-0368
Country USA
 
Platform ID GPL20885
Series (1)
GSE181253 The potent and selective RIPK2 inhibitor BI 706039 improves intestinal inflammation in the TRUC mouse model of Inflammatory Bowel Disease

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
Ager -10.09294059
Alox12 -8.445113558
Alox15 274.9811366
Alox5 78.88971934
Areg 596.3074086
Arg1 19.56794605
Atf2 1496.02097
Bcl2l1 370.5551047
Bcl6 31.1027353
Birc2 543.5769434
C1qa 4447.279191
C1qb 3773.317934
C1ra 541.9291164
C1s 3104.300157
C2 415.0464346
C3 4766.957636
C3ar1 886.3249669
C4a 3783.204896
C6 110.198433
C7 1.441848656

Total number of rows: 248

Table truncated, full table size 4 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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