|
| Status |
Public on Aug 05, 2022 |
| Title |
Liver of WT mouse under CDAA-HSFC diet #2 |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6 x CBA adult male mouse
|
| Organism |
Mus musculus |
| Characteristics |
genotype: WT
|
| Treatment protocol |
Mice were fed with a CDAA-HFSC for 7.5 weeks
|
| Growth protocol |
Mice were housed in a temperature-controlled room (22–24°C) with a 12h dark-light cycle. Animal studies were performed in compliance with European Community specifications regarding the use of laboratory animals and approved by the Nord-Pas de Calais Ethical Committee for animal use (APAFIS#15539-2018053011323354).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol and reverse transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). Total RNA was checked for quantity and quality using the Agilent 2100 Bioanalyzer (Agilent Biotechnologies).
|
| Label |
biotin
|
| Label protocol |
RNA were processed using Affymetrix GeneChip® WT PLUS Reagent Kit.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on MoGene-2_0-st Chip. GeneChips were washed and stained in the Affymetrix Fluidics.
|
| Scan protocol |
Affymetrix GeneChip Scanner 7G and Affymetrix GeneChip Command Console software (AGCC).
|
| Description |
Transcriptomic liver WT mouse
|
| Data processing |
Normalization was performed using the apt-probeset-summarize tool of Affymetrix Power Tools (https://www.affymetrix.com/support/developer/powertools/changelog/index.html) using “gc correction+scale intensity+rma”. Normalization level was set to “Core genes”. The data obtained are log2-transformed.
|
| |
|
| Submission date |
Oct 07, 2021 |
| Last update date |
Aug 05, 2022 |
| Contact name |
Jérôme Eeckhoute |
| E-mail(s) |
jerome.eeckhoute@inserm.fr
|
| Organization name |
CNRS
|
| Lab |
INSERM UMR 1011, Université Lille-Nord de France
|
| Street address |
Bâtiment J&K, Faculté de Médecine de Lille-Pôle Recherche, Boulevard du Professeur Leclerc
|
| City |
Lille |
| ZIP/Postal code |
59045 |
| Country |
France |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE185527 |
Transcriptomic analysis of the livers of Bnc2 heterozygous or wild-type mice fed with a pro-fibrogenic NASH diet |
| GSE185529 |
BNC2 regulated genes in myofibroblasts and liver fibrosis |
|
