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Sample GSM575217 Query DataSets for GSM575217
Status Public on Sep 01, 2010
Title fem-1 oocyte D3 vs fem-1 oocyte D8 array #5 SL12.02.08
Sample type RNA
 
Channel 1
Source name fem-1 D8
Organism Caenorhabditis elegans
Characteristics developmental stage: Day 8 oocytes
genotype: fem-1(hc17)
Biomaterial provider Shijing Luo, Murphy Lab, Lewis-Sigler Institute for Integrative Genomics
Extracted molecule total RNA
Extraction protocol Extraction method : Qiagen Rneasy Kit
Label Cy3
Label protocol Type of label : Cy3
reference URL : http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
Channel 2
Source name fem-1 D3
Organism Caenorhabditis elegans
Characteristics genotype: fem-1(hc17)
developmental stage: Day 3 oocytes
Biomaterial provider Shijing Luo, Murphy Lab, Lewis-Sigler Institute for Integrative Genomics
Extracted molecule total RNA
Extraction protocol Extraction method : Qiagen Rneasy Kit
Label Cy5
Label protocol Type of label : Cy5
reference URL : http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
 
Hybridization protocol reference URL : http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=48835
Scan protocol Pixel Size : 5
Scan Date : 2008-12-02
Scan Time : 10:25:58
Scanner Make : Agilent Technologies Scanner
Scanner Model : G2505B
Scanning software : ChipScan
Scanning software version : A.7.0.1
Description fem-1(hc17) oocyte day8 #7 10.17.08 2nd collection Cy3 vs. fem-1(hc17) oocyte day3 #9 10.12.08 collection Cy5, array #5, SL scanned 12.02.08
Data processing Extraction Software : Agilent Feature Extractor
Extraction Software Version : 9.5.3.1
Datafile type : Agilent result file
 
Submission date Aug 05, 2010
Last update date Aug 06, 2010
Contact name Shijing Luo
E-mail(s) shijingluo@gmail.com
Organization name Princeton University
Street address 160 Carl Icahn Lab, Princeton University
City Princeton
State/province NJ
ZIP/Postal code 08544
Country USA
 
Platform ID GPL7727
Series (2)
GSE23447 Reproductive aging: fem-1 day 3 oocyte vs fem-1 day 8 oocyte
GSE23509 TGF-beta Sma/Mab signaling regulates a set of genes in oocytes associated with reproductive aging and another set in L4 associated with body size growth

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (R_PROCESSED_SIGNAL/G_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 .034 5652.438 5522.159 8648.967 2163.017
2 0 2.367859 4.468402 34.67742 30.12903
3 0 2.349707 4.431367 36.89063 31.76563
4 0 2.333494 4.398988 43.51613 31.20968
5 0 2.322077 4.375596 36.95082 31.81967
6 0 2.309469 4.351689 36.03226 29.67742
7 0 2.302165 4.337123 38.93333 27.53333
8 0 2.294418 4.323281 32.85246 28.70492
9 0 2.290525 4.316437 31.96296 29.14815
10 0 2.286954 4.31143 35.45902 29.68852
11 0 2.286785 4.312439 34.1875 29.01563
12 -.25 2592.039 3082.172 4164.709 1274.855
13 .061 46.75951 44.82863 114.9841 49.36508
14 .211 9.03303 7.802337 54.94643 34.33929
15 .402 12.20979 9.239532 59.91071 34.82143
16 .313 5.422411 4.363864 49.125 30.35938
17 -2.239 261.2334 1233.127 451.2632 520.8421
18 -.196 155.5444 178.1992 284.1212 101.3182
19 .182 2204.862 1943.502 3477.646 794.9385
20 2.221 189.9233 40.73066 335.0781 46.64063

Total number of rows: 44381

Table truncated, full table size 1986 Kbytes.




Supplementary file Size Download File type/resource
GSM575217.txt.gz 13.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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