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Sample GSM575220 Query DataSets for GSM575220
Status Public on Sep 01, 2010
Title fem-1 oocyte D3 vs fem-1 oocyte D8 array #8 SL12.02.08
Sample type RNA
 
Channel 1
Source name fem-1 D3
Organism Caenorhabditis elegans
Characteristics developmental stage: Day 3 oocytes
genotype: fem-1(hc17)
Biomaterial provider Shijing Luo, Murphy Lab, Lewis-Sigler Institute for Integrative Genomics
Extracted molecule total RNA
Extraction protocol Extraction method : Qiagen Rneasy Kit
Label Cy3
Label protocol Type of label : Cy3
reference URL : http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
Channel 2
Source name fem-1 D8
Organism Caenorhabditis elegans
Characteristics genotype: fem-1(hc17)
developmental stage: Day 8 oocytes
Biomaterial provider Shijing Luo, Murphy Lab, Lewis-Sigler Institute for Integrative Genomics
Extracted molecule total RNA
Extraction protocol Extraction method : Qiagen Rneasy Kit
Label Cy5
Label protocol Type of label : Cy5
reference URL : http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=32636
 
 
Hybridization protocol reference URL : http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=48835
Scan protocol Pixel Size : 5
Scan Date : 2008-12-02
Scan Time : 10:25:58
Scanner Make : Agilent Technologies Scanner
Scanner Model : G2505B
Scanning software : ChipScan
Scanning software version : A.7.0.1
Description fem-1(hc17) oocyte day8 #11 10.23.08 2nd collection Cy5 vs. fem-1(hc17) oocyte day3 #20 10.22.08 collection Cy3, array #8, SL scanned 12.02.08
Data processing Extraction Software : Agilent Feature Extractor
Extraction Software Version : 9.5.3.1
Datafile type : Agilent result file
 
Submission date Aug 05, 2010
Last update date Aug 06, 2010
Contact name Shijing Luo
E-mail(s) shijingluo@gmail.com
Organization name Princeton University
Street address 160 Carl Icahn Lab, Princeton University
City Princeton
State/province NJ
ZIP/Postal code 08544
Country USA
 
Platform ID GPL7727
Series (2)
GSE23447 Reproductive aging: fem-1 day 3 oocyte vs fem-1 day 8 oocyte
GSE23509 TGF-beta Sma/Mab signaling regulates a set of genes in oocytes associated with reproductive aging and another set in L4 associated with body size growth

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (G_PROCESSED_SIGNAL/R_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 -0.422 4182.552 3120.772 3864.672 1309.885
2 0 3.653643 3.867199 35.40625 25.67188
3 0 3.687852 3.917773 36.4375 27.14063
4 0 3.718622 3.963971 32.19048 28.7619
5 0 3.749096 4.010684 34.53226 26.80645
6 0 3.777919 4.055835 36.5873 27.90476
7 0 3.805115 4.099291 33.56452 27.33871
8 0 3.830949 4.141543 33.30645 26.82258
9 0 3.854972 4.181706 36.43548 25.80645
10 0 3.877891 4.220908 33.53968 27.95238
11 0 3.899169 4.258261 32.4918 26.88525
12 -0.293 3729.49 3043.653 3189.581 1139.694
13 0.182 58.12877 65.95426 87.13333 53.71667
14 -0.554 18.64493 12.70193 53.90769 34.41538
15 0.635 5.597633 8.693768 43.01613 32.95161
16 2.388 3.983986 20.8599 39.27692 37.23077
17 -0.4 708.0295 536.4126 625.0794 218.6984
18 -0.01 167.0675 165.9594 176.5313 87.92188
19 -0.26 2255.548 1883.241 1897.317 685.5873
20 1.176 83.63682 188.9763 107.3594 95.28125

Total number of rows: 44381

Table truncated, full table size 2029 Kbytes.




Supplementary file Size Download File type/resource
GSM575220.txt.gz 13.3 Mb (ftp)(http) TXT
Processed data included within Sample table
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