|
| Status |
Public on Apr 11, 2022 |
| Title |
Male-blastocyst-1620-2 |
| Sample type |
genomic |
| |
|
| Source name |
Term placenta
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
tissue: Term placenta
sample type: blastocyst
|
| Treatment protocol |
Placentas were collected at E18.5, snap frozen in liquid nitrogen and stored at -80C for DNA extraction.
|
| Growth protocol |
All animals were housed in polysulfone cages containing drinking water and chow (Laboratory Autoclavable Rodent Diet 5010, LabDiet) ad libitum.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from Placentas samples using Phenol:Chloroform according to lab protocols. Then samples were Bisulfite-treated using Qiagen EpiTect Bisulfite Conversion Kit.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Mouse Methylation Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
Sesame R package
|
| |
|
| Submission date |
Jan 07, 2022 |
| Last update date |
Apr 12, 2022 |
| Contact name |
Yemin Lan |
| Organization name |
University of Pennsylvania
|
| Street address |
3400 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL31191 |
| Series (1) |
| GSE193271 |
Placental abnormalities are associated with specific windows of embryo culture in a mouse model |
|
