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| Status |
Public on Apr 11, 2022 |
| Title |
Placental abnormalities are associated with specific windows of embryo culture in a mouse model |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Assisted Reproductive Technologies (ART) employ gamete/embryo handling and culture in vitro to produce offspring. ART pregnancies have increased risk of low birth weight, abnormal placentation, pregnancy complications, and imprinting disorders. We and others have previously shown that embryo culture induces low birth weight, abnormal placental morphology, and lower levels of DNA methylation in placentas in a mouse model of ART. We hypothesized that these adverse effects are linked to a subtle disruption of specific biological processes during preimplantation development. To test this hypothesis, we performed embryo culture for several discrete periods of preimplantation development and assessed fetal and placental outcomes at term. We observed a reduction in fetal:placental ratio in two distinct windows of preimplantation embryo development, while placental morphological abnormalities and reduced imprinting control region methylation were associated with culture prior to the morula stage. We also provide evidence that extended culture to the blastocyst stage induces additional placental DNA methylation changes compared to embryos transferred at the morula stage, and that female concepti exhibited a higher loss of DNA methylation than males. Altogether, this study identifies specific developmental windows of susceptibility and potential targets for embryo culture optimization.
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| Overall design |
Mouse (Mus musculus) term placentas from 1-cell-morula group (n=12, 6 females and 6 males), 1-cell-blastocyst group (n=12, 6 females and 6 males) and Natural group (n=10, 5 females and 5 males) were analyzed.
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| Contributor(s) |
Vrooman LA, Rhon-Calderon EA, Suri KV, Dahiya AK, Lan Y, Schultz RM, Bartolomei MS |
| Citation(s) |
35547813 |
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| Submission date |
Jan 07, 2022 |
| Last update date |
May 25, 2022 |
| Contact name |
Yemin Lan |
| Organization name |
University of Pennsylvania
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| Street address |
3400 Civic Center Blvd
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL31191 |
Infinium MouseMethylation285 BeadChip |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA795897 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE193271_RAW.tar |
174.9 Mb |
(http)(custom) |
TAR (of IDAT) |
| GSE193271_results.csv.gz |
102.3 Mb |
(ftp)(http) |
CSV |
| Processed data included within Sample table |
| Processed data are available on Series record |
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