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Sample GSM5778437 Query DataSets for GSM5778437
Status Public on Apr 11, 2022
Title Male-morula-1109-3
Sample type genomic
 
Source name Term placenta
Organism Mus musculus
Characteristics gender: Male
tissue: Term placenta
sample type: morula
Treatment protocol Placentas were collected at E18.5, snap frozen in liquid nitrogen and stored at -80C for DNA extraction.
Growth protocol All animals were housed in polysulfone cages containing drinking water and chow (Laboratory Autoclavable Rodent Diet 5010, LabDiet) ad libitum.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted and purified from Placentas samples using Phenol:Chloroform according to lab protocols. Then samples were Bisulfite-treated using Qiagen EpiTect Bisulfite Conversion Kit.
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
 
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Mouse Methylation Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Data processing Sesame R package
 
Submission date Jan 07, 2022
Last update date Apr 12, 2022
Contact name Yemin Lan
Organization name University of Pennsylvania
Street address 3400 Civic Center Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL31191
Series (1)
GSE193271 Placental abnormalities are associated with specific windows of embryo culture in a mouse model

Data table header descriptions
ID_REF
VALUE Average Beta

Data table
ID_REF VALUE
cg00101675_BC21 0.651897101
cg00116289_BC21 0.872168962
cg00211372_TC21 0.897489374
cg00531009_BC21 0.79011285
cg00747726_TC21 0.543513217
cg00896209_TC21 0.844287959
cg00910419_BC21 0.082035769
cg00920372_BC21 0.302519163
cg01326836_BC21 0.215407496
cg01340195_BC21 0.695308303
cg01603043_TC21 0.727290934
cg01613817_BC11 0.508070962
cg01683044_BC21 0.076726519
cg02153806_BC21 0.18964691
cg02176373_BC21 0.105775034
cg02187860_TC21 0.811346819
cg02245762_BC11
cg02258130_TC21 0.920694976
cg02380775_BC21 0.792109042
cg02529685_TC11 0.812567602

Total number of rows: 293199

Table truncated, full table size 7094 Kbytes.




Supplementary file Size Download File type/resource
GSM5778437_Male-morula-1109-3_Grn.idat.gz 2.5 Mb (ftp)(http) IDAT
GSM5778437_Male-morula-1109-3_Red.idat.gz 2.6 Mb (ftp)(http) IDAT
Processed data included within Sample table
Processed data are available on Series record

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