|
| Status |
Public on Aug 22, 2023 |
| Title |
Ae.speltoides_grain_20day_Rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Ae.speltoides grain 20day
|
| Organism |
Aegilops speltoides |
| Characteristics |
tissue: grain 20day
cultivar: Y2032
|
| Treatment protocol |
There are 17 tissues of Ae.speltoides, the pistil own 4 treatments and stamen have 5 repeats, the other tissues have 3 treatments. Besides, 7 tissues of wheat, each has 3 treatments.
|
| Growth protocol |
20℃/18℃(day/night) and 16 h/8 h (light/dark).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA
For each sample, total RNA was prepared for sequencing with the Illumina NovaSeq 6000 platform following the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Ae.speltoides_grain_20day_Rep1
|
| Data processing |
Raw reads were trimmed with FastQC and Trimmomatic v0.334 with default parameters. The remaining reads were aligned to the International Wheat Genome Sequencing Consortium (IWGSC) reference genome (version 1.0) using HISAT2 v2.0.5. Gene expression values were computed using StringTie v1.3.3b.
Genome_build: IWGSC RefSeq v1.0
Supplementary_files_format_and_content: FPKM
|
| |
|
| Submission date |
Feb 19, 2022 |
| Last update date |
Aug 22, 2023 |
| Contact name |
Zefu Lu |
| E-mail(s) |
luzefu@live.com
|
| Organization name |
Institute of Crop Sciences
|
| Street address |
No.12 Zhongguancun South St.,Haidian District, Beijing, P.R.China
|
| City |
Beijing |
| State/province |
Haidian |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platform ID |
GPL31956 |
| Series (2) |
| GSE197048 |
The genome sequencing of Sitopsis species provide insights into its contribution to the B subgenome in Triticum aestivum [RNA-seq] |
| GSE197593 |
The genome sequencing of Sitopsis species provide insights into its contribution to the B subgenome in Triticum aestivum |
|
| Relations |
| BioSample |
SAMN26095717 |
| SRA |
SRX14228309 |
