|
Status |
Public on Oct 03, 2022 |
Title |
Taf13-ChEC in Med16-AID, +DMSO, rep-2 |
Sample type |
SRA |
|
|
Source name |
GZY414
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
chec tag: 3xFLAG-MNase background: W303
|
Treatment protocol |
For the AID strains, 3-IAA treated samples were treated with 3-IAA in DMSO at a final concentration of 0.5mM for 30 minutes. DMSO-treated samples were treated with DMSO (1:2000 by culture volume) for 30 minutes
|
Growth protocol |
Cells were grown at 30℃ in YPD to an OD600 of 0.7-1.0
|
Extracted molecule |
genomic DNA |
Extraction protocol |
ChEC performed as described in Tourigny et al. 2018, PMID: 30275344 NEB Next Ultra II DNA Library Prep Kit for Illumina ChEC-seq
|
|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Alignment with Bowtie2 (default settings plus "--no-unal --dovetail –no-discordant --no-mixed") HOMER makeTagDirectory HOMER makeUCSCfile ("-bigWig -norm 1e6") Assembly: sacCer3 Supplementary files format and content: bigWig
|
|
|
Submission date |
Jul 01, 2022 |
Last update date |
Oct 03, 2022 |
Contact name |
Moustafa Saleh |
E-mail(s) |
moumsaleh@gmail.com
|
Organization name |
Indiana University
|
Department |
Biology
|
Lab |
Zentner
|
Street address |
915 E 3rd St
|
City |
Bloomington |
State/province |
IN |
ZIP/Postal code |
47405 |
Country |
USA |
|
|
Platform ID |
GPL19756 |
Series (2) |
GSE207369 |
Involvement of the SAGA and TFIID coactivator complexes in transcriptional dysregulation caused by separation of core and tail Mediator modules (ChEC-Seq) |
GSE207371 |
Involvement of the SAGA and TFIID coactivator complexes in transcriptional dysregulation caused by separation of core and tail Mediator modules |
|
Relations |
BioSample |
SAMN29473188 |
SRA |
SRX15967463 |