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| Status |
Public on Oct 03, 2022 |
| Title |
Involvement of the SAGA and TFIID coactivator complexes in transcriptional dysregulation caused by separation of core and tail Mediator modules (ChEC-Seq) |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Regulation of RNA polymerase II (RNAPII) transcription requires the concerted efforts of several multisubunit coactivator complexes, which interact with the RNAPII preinitiation complex (PIC) to stimulate transcription. We previously showed that separation of the Mediator core (cMed) from Mediator’s tail module results in modest overactivation of genes annotated as highly dependent on TFIID for expression. However, it is unclear if other coactivators are involved in this phenomenon. Here, we show that the overactivation of certain genes by cMed/tail separation is blunted by disruption of the SAGA complex through removal of its structural Spt20 subunit, though this downregulation does not appear to depend on reduced SAGA association with the genome. Consistent with the enrichment of TFIID-dependent genes among genes overactivated by cMed/tail separation, depletion of the essential TFIID subunit Taf13 suppressed overactivation of these genes when Med16 was simultaneously removed. As with SAGA, this effect did not appear to be dependent on reduced genomic association of TFIID. Given that the observed changes in gene expression could not be clearly linked to alterations in SAGA or TFIID occupancy, our data may suggest that the cMed/tail connection is important for modulation of SAGA and/or TFIID conformation and/or function at target genes.
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| Overall design |
ChEC-seq of SAGA and TFIID subunits in Med16 deletion or depletion strains.
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| Contributor(s) |
Saleh MM, Zentner GE |
| Citation(s) |
36331351 |
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| Submission date |
Jul 01, 2022 |
| Last update date |
Jan 02, 2023 |
| Contact name |
Moustafa Saleh |
| E-mail(s) |
moumsaleh@gmail.com
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| Organization name |
Indiana University
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| Department |
Biology
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| Lab |
Zentner
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| Street address |
915 E 3rd St
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| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
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| Platforms (1) |
| GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
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| Samples (22)
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| GSM6285151 |
Spt3 in WT, rep-1 |
| GSM6285152 |
Spt3 in WT, rep-2 |
| GSM6285153 |
Spt3 in WT, rep-3 |
| GSM6285154 |
Spt8 in med16-deletion, rep-1 |
| GSM6285155 |
Spt8 in med16-deletion, rep-2 |
| GSM6285156 |
Spt8 in WT, rep-1 |
| GSM6285157 |
Spt8 in WT, rep-2 |
| GSM6285158 |
Taf13-ChEC in Med16-AID, +DMSO, rep-1 |
| GSM6285159 |
Taf13-ChEC in Med16-AID, +DMSO, rep-2 |
| GSM6285160 |
Taf13-ChEC in Med16-AID, +IAA, rep-1 |
| GSM6285161 |
Taf13-ChEC in Med16-AID, +IAA, rep-2 |
| GSM6285162 |
Taf1-ChEC in Med16-AID, +DMSO, rep-1 |
| GSM6285163 |
Taf1-ChEC in Med16-AID, +DMSO, rep-2 |
| GSM6285164 |
Taf1-ChEC in Med16-AID, +IAA, rep-1 |
| GSM6285165 |
Taf1-ChEC in Med16-AID, +IAA, rep-2 |
| GSM6285166 |
Spt3-ChEC in Spt20-AID, +DMSO, rep-1 |
| GSM6285167 |
Spt3-ChEC in Spt20-AID, +DMSO, rep-2 |
| GSM6285168 |
Spt3-ChEC in Spt20-AID, +IAA, rep-1 |
| GSM6285169 |
Spt3-ChEC in Spt20-AID, +IAA, rep-2 |
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| This SubSeries is part of SuperSeries: |
| GSE207371 |
Involvement of the SAGA and TFIID coactivator complexes in transcriptional dysregulation caused by separation of core and tail Mediator modules |
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| Relations |
| BioProject |
PRJNA854939 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE207369_RAW.tar |
212.0 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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