|
| Status |
Public on Oct 03, 2022 |
| Title |
Taf13-ChEC in Med16-AID, +IAA, rep-2 |
| Sample type |
SRA |
| |
|
| Source name |
GZY414
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
chec tag: 3xFLAG-MNase
background: W303
|
| Treatment protocol |
For the AID strains, 3-IAA treated samples were treated with 3-IAA in DMSO at a final concentration of 0.5mM for 30 minutes. DMSO-treated samples were treated with DMSO (1:2000 by culture volume) for 30 minutes
|
| Growth protocol |
Cells were grown at 30℃ in YPD to an OD600 of 0.7-1.0
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChEC performed as described in Tourigny et al. 2018, PMID: 30275344
NEB Next Ultra II DNA Library Prep Kit for Illumina
ChEC-seq
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Alignment with Bowtie2 (default settings plus "--no-unal --dovetail –no-discordant --no-mixed")
HOMER makeTagDirectory
HOMER makeUCSCfile ("-bigWig -norm 1e6")
Assembly: sacCer3
Supplementary files format and content: bigWig
|
| |
|
| Submission date |
Jul 01, 2022 |
| Last update date |
Oct 03, 2022 |
| Contact name |
Moustafa Saleh |
| E-mail(s) |
moumsaleh@gmail.com
|
| Organization name |
Indiana University
|
| Department |
Biology
|
| Lab |
Zentner
|
| Street address |
915 E 3rd St
|
| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
| |
|
| Platform ID |
GPL19756 |
| Series (2) |
| GSE207369 |
Involvement of the SAGA and TFIID coactivator complexes in transcriptional dysregulation caused by separation of core and tail Mediator modules (ChEC-Seq) |
| GSE207371 |
Involvement of the SAGA and TFIID coactivator complexes in transcriptional dysregulation caused by separation of core and tail Mediator modules |
|
| Relations |
| BioSample |
SAMN29473186 |
| SRA |
SRX15967465 |
