|
| Status |
Public on Feb 08, 2023 |
| Title |
WT_N2_1 |
| Sample type |
SRA |
| |
|
| Source name |
WT_N2_1
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: wild type (WT)
developmental stage: boyes: 5.10
tissue: rosette
|
| Treatment protocol |
N2-abiotic stress-nitrogen-nitrate nutritionquantity 2mM
|
| Growth protocol |
Media=poting soil,Hygrometry 50%,24 day and 19°C night, 100 µmol.m-2.s-1 Light
|
| Extracted molecule |
total RNA |
| Extraction protocol |
WT_N2_1(100mg)
rna_selection:selection polyA(3'seq), single, stranded:yes, Protocol:PT_QuantSEQ-3_prime_mRNA_simple_indexage_B_17-11-21.docx
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Genome_build: genome of Ath TAIR10
genome mapping and counts of reads by gene locus are done with Tophat2/HTSeqcount; exact multihits are removed and count only consistent PE reads (in PE case).
Supplementary_files_format_and_content: type:count file (txt); content:GeneID,counts
|
| |
|
| Submission date |
Jul 13, 2022 |
| Last update date |
Feb 08, 2023 |
| Contact name |
Véronique BRUNAUD |
| E-mail(s) |
veronique.brunaud@inrae.fr
|
| Organization name |
INRAE - CNRS - UPSUD
|
| Lab |
IPS2
|
| Street address |
rue Noetzlin
|
| City |
Gif-sur-Yvette |
| ZIP/Postal code |
91190 |
| Country |
France |
| |
|
| Platform ID |
GPL19580 |
| Series (1) |
| GSE208151 |
Protein farnesylation is involved in the adaptation of Arabidopsis to nitrogen starvation |
|
| Relations |
| BioSample |
SAMN29720031 |
| SRA |
SRX16203553 |
