|
| Status |
Public on Apr 24, 2024 |
| Title |
E12.5 Male IVF+TEBx Placenta 6 |
| Sample type |
genomic |
| |
|
| Source name |
E12.5 Placenta Male IVF+TEBx 6
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
group: IVF+TEBx
tissue: placenta
age: E12.5
|
| Treatment protocol |
Placentas were collected at E182.5, snap frozen in liquid nitrogen and stored at -80C for DNA extraction.
|
| Growth protocol |
All animals were housed in polysulfone cages containing drinking water and chow (Laboratory Irradiated Rodent Diet 5LG4, LabDiet) ad libitum.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from Placentas samples using Phenol:Chloroform according to lab protocols. Then samples were Bisulfite-treated using Qiagen EpiTect Bisulfite Conversion Kit.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol:
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented, and hybridized to Illumina Infinium Mouse285 Methylation BeadChip 12v1-0 using standard Illumina Protocol
|
| Scan protocol |
Illumina iScan System
|
| Description |
Bisulfite treated
Male-IVFTEBx-B2B
Genomic DNA from E12.5 Male IVF+TEBx Placenta 6
|
| Data processing |
SeSAMe R Package
Beta values of individual probe for each sample. Probes that did not pass SeSAMe quality control pOOBAH approach are masked with NA
|
| |
|
| Submission date |
Feb 08, 2023 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Eric Alejandro Rhon Calderon |
| E-mail(s) |
eric.rhoncalderon@pennmedicine.upenn.edu
|
| Phone |
2152909019
|
| Organization name |
University of Pennsylvania
|
| Department |
Cell and Developmental Biology
|
| Lab |
Bartolomei Lab
|
| Street address |
3400 Civic Center Blvd, Bldg 421, Floor 9-175
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL31191 |
| Series (2) |
| GSE224844 |
Trophectoderm biopsies of blastocysts for preimplantation genetic testing following in vitro fertilization and embryo culture increases epigenetic dysregulation in a mouse model [array] |
| GSE225318 |
Trophectoderm biopsies of blastocysts for preimplantation genetic testing following in vitro fertilization and embryo culture increases epigenetic dysregulation in a mouse model |
|
