|
| Status |
Public on May 24, 2024 |
| Title |
DMSO_H3K27AC |
| Sample type |
SRA |
| |
|
| Source name |
brain
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: brain
cell line: primary iOLs
cell type: oligodendrocytes
genotype: wildtype
treatment: DMSO
antibody: H3K27AC
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cut &Run cleaved fragments were purified by Qiagen MinElute PCR Purification Kit.
Libraries were prepared for Illumina sequencing with Library Prep for CUT&RUN with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7645).
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
All sequencing data were mapped to rat genome rn5 and peak calling was performed using MACS (Model-based Analysis of ChIP-Seq) version 1.4.2 (http://liulab.dfci.harvard.edu/MACS) with a P value cutoff of 1 x e-9 to obtain raw binding peaks
Assembly: rn5
Supplementary files format and content: wig,bigwig
Library strategy: Cut&Run
|
| |
|
| Submission date |
May 19, 2023 |
| Last update date |
May 24, 2024 |
| Contact name |
XUEZHAO LIU |
| E-mail(s) |
xuezhao.liu@cchmc.org
|
| Organization name |
cincinnati children's hospital medical center
|
| Street address |
3333 Burnet Ave. MLC 7013, T6.525
|
| City |
cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL25029 |
| Series (2) |
| GSE232953 |
Small-molecule-induced epigenetic rejuvenation overcomes myelinogenic barriers and stimulates myelin repair [Cut&Run] |
| GSE232956 |
Small-molecule-induced epigenetic rejuvenation overcomes myelinogenic barriers and stimulates myelin repair |
|
| Relations |
| BioSample |
SAMN35220431 |
| SRA |
SRX20454138 |
