|
| Status |
Public on Jun 22, 2011 |
| Title |
Brain microglia-2 |
| Sample type |
RNA |
| |
|
| Source name |
Brain microglia
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: female
age: 6-8 weeks
source tissue: brain
cell phenotype: Lin- (CD19-CD3-NK1.1-B220-Ter119-) CD45intCD11c-
cell type: microglia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Mini prep extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip MOE-430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner
|
| Data processing |
The data were analyzed with Genespring, using default RMA normalization. The normalized values are log2 scaled, where the median is set as zero.
|
| |
|
| Submission date |
Jun 13, 2011 |
| Last update date |
Apr 10, 2013 |
| Contact name |
Kang Liu |
| E-mail(s) |
kl2529@columbia.edu
|
| Phone |
212 305-0839
|
| Fax |
212 305-0839
|
| Organization name |
Columbia University
|
| Department |
Microbiology
|
| Lab |
Liu
|
| Street address |
701 W. 168 ST., HHSC Rm. 1302
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE29949 |
Gene expression comparison among spleen dendritic cells, brain microglia, brain dendritic cells and bone marrow monocytes |
|
| Relations |
| Reanalyzed by |
GSE45704 |
