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Sample GSM741201 Query DataSets for GSM741201
Status Public on Jun 22, 2011
Title Brain DC-2
Sample type RNA
 
Source name Brain DC
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
age: 6-8 weeks
source tissue: brain
cell phenotype: Lin-CD45hiCD11c+.
cell type: dendritic cell
Treatment protocol Mice received subcutaneous 1 million B16-Flt3L tumor inoculation 12 days before organ harvest.
Extracted molecule total RNA
Extraction protocol Qiagen Mini prep extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip MOE-430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner
Data processing The data were analyzed with Genespring, using default RMA normalization was used. The normalized values are log2 scaled, where the median is set as zero.
 
Submission date Jun 13, 2011
Last update date Apr 10, 2013
Contact name Kang Liu
E-mail(s) kl2529@columbia.edu
Phone 212 305-0839
Fax 212 305-0839
Organization name Columbia University
Department Microbiology
Lab Liu
Street address 701 W. 168 ST., HHSC Rm. 1302
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL1261
Series (1)
GSE29949 Gene expression comparison among spleen dendritic cells, brain microglia, brain dendritic cells and bone marrow monocytes
Relations
Reanalyzed by GSE45704

Data table header descriptions
ID_REF
VALUE Value

Data table
ID_REF VALUE
AFFX-BioB-5_at 1.0111732
AFFX-BioB-M_at 1.0550299
AFFX-BioB-3_at 0.96328735
AFFX-BioC-5_at 0.96809196
AFFX-BioC-3_at 1.0975351
AFFX-BioDn-5_at 0.68826103
AFFX-BioDn-3_at 0.20401573
AFFX-CreX-5_at 0.10587978
AFFX-CreX-3_at 0.068097115
AFFX-DapX-5_at 2.965374
AFFX-DapX-M_at 2.0265179
AFFX-DapX-3_at 1.3562794
AFFX-LysX-5_at 3.3624744
AFFX-LysX-M_at 2.634387
AFFX-LysX-3_at 1.9330969
AFFX-PheX-5_at 2.2701511
AFFX-PheX-M_at 2.8686943
AFFX-PheX-3_at 2.0619965
AFFX-ThrX-5_at 2.1551547
AFFX-ThrX-M_at 3.474186

Total number of rows: 45101

Table truncated, full table size 1001 Kbytes.




Supplementary file Size Download File type/resource
GSM741201.CEL.gz 5.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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