|
| Status |
Public on Oct 30, 2012 |
| Title |
WT TAP |
| Sample type |
SRA |
| |
|
| Source name |
Young adult C. elegans
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: Bristol N2 background
developmental stage: Young adult C. elegans
rna treatment: TAP treated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using fast protK mediated lysis and Trizol LS protocols. RNA was then treated with Tobacco Acid Pyrophosphatase according manufactorers conditions. 5' and 3' adapttors were ligated.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Small RNA sequence data
5' barcode TACA, 3' adapter TCGTATGCCGTCTTCTGCTTG
|
| Data processing |
3' adapter sequences were trimmed and inserts longer than 18 nt were mapped to the C. elegans genome (WS220)
|
| |
|
| Submission date |
Aug 31, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Eugene Berezikov |
| E-mail(s) |
e.berezikov@umcg.nl
|
| Phone |
+31 50 361 73 00
|
| Organization name |
University Medical Center Groningen
|
| Department |
European Research Institute for the Biology of Ageing
|
| Street address |
Antonius Deusinglaan 1
|
| City |
Groningen |
| ZIP/Postal code |
9713AV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL9269 |
| Series (1) |
|
| Relations |
| SRA |
SRX095336 |
| BioSample |
SAMN00715030 |
