|
| Status |
Public on Jun 01, 2024 |
| Title |
A2AM31 |
| Sample type |
protein |
| |
|
| Source name |
monoclonal antibody
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Antibody
|
| Treatment protocol |
5 ug/ml
|
| Growth protocol |
N/A
|
| Extracted molecule |
protein |
| Extraction protocol |
MAb expressed in HEK293 cell
|
| Label |
Alexa 647
|
| Label protocol |
Alexa 647
|
| |
|
| Hybridization protocol |
Arrays were blocked with 3% BSA overnight, and diluted samples was incubated on the array for 4 hours at 37 C and gentle agitation (100 RPM). Slides were then washed with PBS contain 0.05% Tween (PBST). After washing, bound antibodies were detected by Alexa 647-labeled goat anti-human IgG (Jackson Immunoresearch). Slides were then washed with PBST, spun dry in a centrifuge, and scanned.
|
| Scan protocol |
The GenePix 4000 Microarray scanner system (Molecular Devices, CA) was used for scanning.
|
| Description |
IgG
D01
|
| Data processing |
We analyzed images using GenePix Pro 7.3.0.0 to measure median pixel intensity (MPI) and neighboring background pixel intensity (BPI) of individual spots. The median fluorescence intensity (MFI), representing AM-epitope specific reactivity, was calculated using the MPI minus the BPI and averaged from the triplicate spots. A minimun value was set at 100.
|
| |
|
| Submission date |
Apr 08, 2024 |
| Last update date |
Jun 01, 2024 |
| Contact name |
Jacqueline Achkar |
| E-mail(s) |
jacqueline.achkar@einsteinmed.edu
|
| Organization name |
Albert Einstein College of Medicine
|
| Street address |
1300 Morris Park Ave
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL34369 |
| Series (1) |
| GSE263497 |
Deciphering Mycobacterium tuberculosis glycan epitopes through clustering of human polyclonal and isolating novel monoclonal antibodies II |
|
