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Platform GPL34369 Query DataSets for GPL34369
Status Public on Jun 01, 2024
Title LAM Subarray 30 - Antigens
Technology type spotted peptide or protein
Distribution non-commercial
Organism synthetic construct
Manufacturer Todd Lowary, University of Alberta and Engineering Arts Tempe Arizona
Manufacture protocol Microarray slides were printed at Engineering Arts LLC (Phoenix, AZ). BSA conjugates were noncontact printed on Schott type E slides (Schott North America, Inc., Elmsford, NY) using an Engineering Arts au301 Rainmaker microarray printer and dispensed at 100 μg/mL in buffer (1:10 phosphate-buffered saline plus 0.005% vol/vol Triton X-100). Antigens were dispensed at 360 pL per spot to produce 140- to 160-m diameter spots in triplicate. Relative humidity was maintained at 65%; slides were subjected to white light for 100% inline drop inspection. Printing was carried out in a HEPA-filtered environment at room temperature. Printed slides were stored at –20 °C until use. Each slide contains 24 identical array containing 30 components printed in duplicate.
 
 
Submission date Apr 08, 2024
Last update date Jun 01, 2024
Contact name Jacqueline Achkar
E-mail(s) jacqueline.achkar@einsteinmed.edu
Organization name Albert Einstein College of Medicine
Street address 1300 Morris Park Ave
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
 
Samples (118) GSM8193552, GSM8193553, GSM8193554, GSM8193555, GSM8193556, GSM8193557 
Series (2)
GSE263496 Deciphering Mycobacterium tuberculosis glycan epitopes through clustering of human polyclonal and isolating novel monoclonal antibodies I
GSE263497 Deciphering Mycobacterium tuberculosis glycan epitopes through clustering of human polyclonal and isolating novel monoclonal antibodies II

Data table header descriptions
ID
SPOT_ID
SAMPLE NUMBER
Structure
Linker
Loading on BSA

Data table
ID SPOT_ID SAMPLE NUMBER Structure Linker Loading on BSA
1 S#1 YB-8-99 beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 14
2 S#2 YB-8-101 alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 14
3 S#3 YB-8-111 alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyltyl Squarate 11
4 S#4 YB-8-113 alpha-D-Manp-(1?2)-alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 12
5 S#5 YB-8-123 alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 12
6 S#6 YB-8-125 alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 12
7 S#7 YB-8-133 5-deoxy-5-thiomethyl-alpha-D-Xylf-(1?4)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 11
8 S#8 YB-8-141 5-deoxy-5-thiomethyl-alpha-D-Xylf-(1?4)-alpha-D-Manp-(1?2)-alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 11
9 S#9 YB-8-143 5-deoxy-5-thiomethyl-alpha-D-Xylf-(1?4)-alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 8
10 S#10 YB-8-149 beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[5-deoxy-5-thiomethyl-alpha-D-Xylf-(1?4)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 13
11 S#11 YB-8-151 5-deoxy-5-thiomethyl-alpha-D-Xylf-(1?4)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 9
12 S#12 YB-11-151 alpha-D-Manp-(1?5)-[beta-D-Araf-(1?2)]-alpha-D-Araf-(1?5)-[alpha-D-Manp-(1?5)-[beta-D-Araf-(1?2)]-alpha-D-Araf-(1?3)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 15
13 S#15 YB-BSA-03 beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 8
14 S#16 YB-BSA-04 beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 8
15 S#17 YB-BSA-05 alpha-D-Manp-(1®6)-[alpha-D-Manp-(1®3)]-alpha-D-Manp-(1®6)-alpha-D-Manp-8?-aminooctyl Squarate 20
16 S#18 YB-BSA-06 beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 7
17 S#19 YB-BSA-07 alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-[alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?3)]-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 2
18 S#20 YB-BSA-08 beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate
19 S#21 YB-BSA-09 5-O-{alpha-D-Araf-(1?3)-[alpha-D-Araf-(1?5)]-alpha-D-Araf}-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?3)-[5-O-{alpha-D-Araf-(1?3)-[alpha-D-Araf-(1?5)]-alpha-D-Araf}-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-Araf-(1?5)]-alpha-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 5
20 S#22 YB-BSA-10 5-O-{beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf}-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?3)-[5-O-{beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf}-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl Squarate 3

Total number of rows: 30

Table truncated, full table size 5 Kbytes.




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