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Status
Public on Jun 01, 2024
Title
LAM Subarray 30 - Antigens
Technology type
spotted peptide or protein
Distribution
non-commercial
Organism
synthetic construct
Manufacturer
Todd Lowary, University of Alberta and Engineering Arts Tempe Arizona
Manufacture protocol
Microarray slides were printed at Engineering Arts LLC (Phoenix, AZ). BSA conjugates were noncontact printed on Schott type E slides (Schott North America, Inc., Elmsford, NY) using an Engineering Arts au301 Rainmaker microarray printer and dispensed at 100 μg/mL in buffer (1:10 phosphate-buffered saline plus 0.005% vol/vol Triton X-100). Antigens were dispensed at 360 pL per spot to produce 140- to 160-m diameter spots in triplicate. Relative humidity was maintained at 65%; slides were subjected to white light for 100% inline drop inspection. Printing was carried out in a HEPA-filtered environment at room temperature. Printed slides were stored at –20 °C until use. Each slide contains 24 identical array containing 30 components printed in duplicate.
Submission date
Apr 08, 2024
Last update date
Jun 01, 2024
Contact name
Jacqueline Achkar
E-mail(s)
jacqueline.achkar@einsteinmed.edu
Organization name
Albert Einstein College of Medicine
Street address
1300 Morris Park Ave
City
Bronx
State/province
NY
ZIP/Postal code
10461
Country
USA
Samples (118)
GSM8193552 , GSM8193553 , GSM8193554 , GSM8193555 , GSM8193556 , GSM8193557
GSM8193558 ,
GSM8193559 ,
GSM8193560 ,
GSM8193561 ,
GSM8193562 ,
GSM8193563 ,
GSM8193564 ,
GSM8193565 ,
GSM8193566 ,
GSM8193567 ,
GSM8193568 ,
GSM8193569 ,
GSM8193570 ,
GSM8193571 ,
GSM8193572 ,
GSM8193573 ,
GSM8193574 ,
GSM8193575 ,
GSM8193576 ,
GSM8193577 ,
GSM8193578 ,
GSM8193579 ,
GSM8193580 ,
GSM8193581 ,
GSM8193582 ,
GSM8193583 ,
GSM8193584 ,
GSM8193585 ,
GSM8193586 ,
GSM8193587 ,
GSM8193588 ,
GSM8193589 ,
GSM8193590 ,
GSM8193591 ,
GSM8193592 ,
GSM8193593 ,
GSM8193594 ,
GSM8193595 ,
GSM8193596 ,
GSM8193597 ,
GSM8193598 ,
GSM8193599 ,
GSM8193600 ,
GSM8193601 ,
GSM8193602 ,
GSM8193603 ,
GSM8193604 ,
GSM8193605 ,
GSM8193606 ,
GSM8193607 ,
GSM8193608 ,
GSM8193609 ,
GSM8193610 ,
GSM8193611 ,
GSM8193612 ,
GSM8193613 ,
GSM8193614 ,
GSM8193615 ,
GSM8193616 ,
GSM8193617 ,
GSM8193618 ,
GSM8193619 ,
GSM8193620 ,
GSM8193621 ,
GSM8193622 ,
GSM8193623 ,
GSM8193624 ,
GSM8193625 ,
GSM8193626 ,
GSM8193627 ,
GSM8193628 ,
GSM8193629 ,
GSM8193630 ,
GSM8193631 ,
GSM8193632 ,
GSM8193633 ,
GSM8193634 ,
GSM8193635 ,
GSM8193636 ,
GSM8193637 ,
GSM8193638 ,
GSM8193639 ,
GSM8193640 ,
GSM8193641 ,
GSM8193642 ,
GSM8193643 ,
GSM8193644 ,
GSM8193645 ,
GSM8193646 ,
GSM8193647 ,
GSM8193648 ,
GSM8193649 ,
GSM8193650 ,
GSM8193651 ,
GSM8193652 ,
GSM8193653 ,
GSM8193654 ,
GSM8193655 ,
GSM8193656 ,
GSM8193657 ,
GSM8193658 ,
GSM8193659 ,
GSM8193660 ,
GSM8193661 ,
GSM8193662 ,
GSM8193663 ,
GSM8193664 ,
GSM8193665 ,
GSM8193666 ,
GSM8193667 ,
GSM8193668 ,
GSM8193669
Series (2)
GSE263496
Deciphering Mycobacterium tuberculosis glycan epitopes through clustering of human polyclonal and isolating novel monoclonal antibodies I
GSE263497
Deciphering Mycobacterium tuberculosis glycan epitopes through clustering of human polyclonal and isolating novel monoclonal antibodies II
Data table header descriptions
ID
SPOT_ID
SAMPLE NUMBER
Structure
Linker
Loading on BSA
Data table
ID
SPOT_ID
SAMPLE NUMBER
Structure
Linker
Loading on BSA
1
S#1
YB-8-99
beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
14
2
S#2
YB-8-101
alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
14
3
S#3
YB-8-111
alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyltyl
Squarate
11
4
S#4
YB-8-113
alpha-D-Manp-(1?2)-alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
12
5
S#5
YB-8-123
alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
12
6
S#6
YB-8-125
alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
12
7
S#7
YB-8-133
5-deoxy-5-thiomethyl-alpha-D-Xylf-(1?4)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
11
8
S#8
YB-8-141
5-deoxy-5-thiomethyl-alpha-D-Xylf-(1?4)-alpha-D-Manp-(1?2)-alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
11
9
S#9
YB-8-143
5-deoxy-5-thiomethyl-alpha-D-Xylf-(1?4)-alpha-D-Manp-(1?2)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
8
10
S#10
YB-8-149
beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[5-deoxy-5-thiomethyl-alpha-D-Xylf-(1?4)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
13
11
S#11
YB-8-151
5-deoxy-5-thiomethyl-alpha-D-Xylf-(1?4)-alpha-D-Manp-(1?5)-beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
9
12
S#12
YB-11-151
alpha-D-Manp-(1?5)-[beta-D-Araf-(1?2)]-alpha-D-Araf-(1?5)-[alpha-D-Manp-(1?5)-[beta-D-Araf-(1?2)]-alpha-D-Araf-(1?3)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
15
13
S#15
YB-BSA-03
beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
8
14
S#16
YB-BSA-04
beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
8
15
S#17
YB-BSA-05
alpha-D-Manp-(1®6)-[alpha-D-Manp-(1®3)]-alpha-D-Manp-(1®6)-alpha-D-Manp-8?-aminooctyl
Squarate
20
16
S#18
YB-BSA-06
beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
7
17
S#19
YB-BSA-07
alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-[alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?3)]-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
2
18
S#20
YB-BSA-08
beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
19
S#21
YB-BSA-09
5-O-{alpha-D-Araf-(1?3)-[alpha-D-Araf-(1?5)]-alpha-D-Araf}-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?3)-[5-O-{alpha-D-Araf-(1?3)-[alpha-D-Araf-(1?5)]-alpha-D-Araf}-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-Araf-(1?5)]-alpha-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
5
20
S#22
YB-BSA-10
5-O-{beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf}-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?3)-[5-O-{beta-D-Araf-(1?2)-alpha-D-Araf-(1?3)-[beta-D-Araf-(1?2)-alpha-D-Araf-(1?5)]-alpha-D-Araf}-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)]-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-(1?5)-alpha-D-Araf-8?-aminooctyl
Squarate
3
Total number of rows: 30 Table truncated, full table size 5 Kbytes .
Supplementary data files not provided