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| Status |
Public on Jun 01, 2024 |
| Title |
Deciphering Mycobacterium tuberculosis glycan epitopes through clustering of human polyclonal and isolating novel monoclonal antibodies II |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
|
| Summary |
The immunomodulatory mycobacterial surface antigen lipoarabinomannan (LAM) with its immunogenic glycan component arabinomannan (AM) facilitates Mycobacterium tuberculosis’ (Mtb) escape from the host’s immune response. Some but not all antibodies against AM can protect against TB. To better understand which of AM’s structures to target, we must first identify the spectrum of its epitopes.
Through isolating and characterizing a panel of novel human mAbs with binding to distinct AM OS motifs, we further defined the glycan epitope structures, identified a new epitope, and determined their differences among mycobacterial strains.
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| Overall design |
Monoclonal antibodies at 5 ug/ml were tested for direct binding to 30 synthetic oligosaccharides
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| Contributor(s) |
Chen T, Liu Y, Lowary TL, Achkar JM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 08, 2024 |
| Last update date |
Jun 01, 2024 |
| Contact name |
Jacqueline Achkar |
| E-mail(s) |
jacqueline.achkar@einsteinmed.edu
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| Organization name |
Albert Einstein College of Medicine
|
| Street address |
1300 Morris Park Ave
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA1097646 |
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