GEO DataSets

(Submitter supplied) In mammalian somatic cells, the relative contribution of RNAi and the type I interferon response during viral infection is unclear. The apparent inefficiency of antiviral RNAi might be due to self-limiting properties and mitigating co-factors of the key enzyme Dicer. In particular, the helicase domain of human Dicer appears to be an important restriction factor of its activity. Here, we study the involvement of several helicase-truncated mutants of human Dicer in the antiviral response. more...

Organism:

Homo sapiens; Sindbis virus

Type:

Other

Platform:

GPL33716

8 Samples

Download data: BW, TXT

(Submitter supplied) In mammalian somatic cells, the relative contribution of RNAi and the type I interferon response during viral infection is unclear. The apparent inefficiency of antiviral RNAi might be due to self-limiting properties and mitigating co-factors of the key enzyme Dicer. In particular, the helicase domain of human Dicer appears to be an important restriction factor of its activity. Here, we study the involvement of several helicase-truncated mutants of human Dicer in the antiviral response. more...

Organism:

Homo sapiens; Sindbis virus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33716 GPL20301

18 Samples

Download data: TXT

(Submitter supplied) Purpose: Next-generation sequencing (NGS) on targeted locus in Sindbis genome to determine frequency changes of artificial microRNAs expressed by viruses after passaging in cancer and normal cells Methods: RNA was harvested in Trizol 488 (Thermo Fisher). RNA was extracted using the manufacturer’s protocol and quantified by nanodrop. Sequencing was done by SeqMatic on a MiSeq v3 platform generating 75bp reads. more...

Organism:

Sindbis virus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23568

21 Samples

Download data: TXT

(Submitter supplied) Sindbis virus is a mutagenic ssRNA arbor virus. The structural genome component of this virus can be exchanged for any transgene of < 6kb in size. Here we sequence transgenic elements inserted to the virus. Our EGFP transgene was analyzed as a function of time during viral replication to quantify the mutagenic rate of the virus. Our nanobody transgene was analyzed to assess cDNA library complexity and identity.

Organism:

Sindbis virus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25880

22 Samples

Download data: CSV, FASTA, PY, TXT, XLSX

(Submitter supplied) The role of RNA silencing as a defense mechanism against viruses remains to be formerly established in mammalian somatic cells. Here, we determined the antiviral properties of human and Drosophila Dicer proteins in a heterologous setup. We expressed human Dicer (hDicer) in Drosophila, and Drosophila Dicer-2 in human cells, and measured the impact on the response to Sindbis virus (SINV) infection. In flies, hDicer presents a low processing activity, but partially rescues a Dcr2 null mutation in flies. more...

Organism:

Homo sapiens; Sindbis virus; Drosophila melanogaster

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL19381 GPL19380

11 Samples

Download data: BED, TXT