GEO DataSets

(Submitter supplied) Human cardiovascular stem cells were isolated from adult (57-75 year old) and neonatal (<1 month old) atrial tissue. Cardiovascular stem cells were then cloned by single cell dilution and compared using sabiosciences cell development & differentiation miRNA PCR array.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL15085

3 Samples

Download data: TXT

(Submitter supplied) Skin specimens were derived from involved skin of 3 systemic scleroderma (SSc), 3 localized scleroderma (LSc) and 3 keloid patients. These skin samples and 3 control skins were collected and fixed in formaldehyde immediately after resections. The microRNA (miRNA) isolation from human skin tissue was performed using miRNeasy FFPE kit (Qiagen). For PCR array, miRNAs were reverse-transcribed into first strand cDNA using RT2 miRNA First Strand Kit (SABiosciences). more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL15085

4 Samples

Download data: TXT

(Submitter supplied) Normal fibroblasts and SSc fibroblasts between the third and six subpassages were used for experiments. Normal and scleroderma fibroblasts were serum-starved for 24 hours and incubated in the presence or absence of TGF-β1 (2ng/ml) for 6 hours. Total RNA was extracted from culture cells with ISOGEN (Nippon Gene, Tokyo, Japan). MicroRNA isolation from total RNA was performed using RT2 qPCR-Grade miRNA Isolation Kit (SA Bioscience). more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL15085

3 Samples

Download data: TXT

(Submitter supplied) PCR assay Protocol: see manufacturer's website

Organism:

Homo sapiens

3 Series

10 Samples

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