GEO DataSets

(Submitter supplied) A total of 20 KYSE human esophageal squamous cell carcinoma cell lines (KYSE-30, -140, -150, -170, -180, -200, -220, -350, -410, -450, -510, -520, -590, -770, -850, -890, -1170, -1190, -1250, and -2270) and a non-cancerous esophageal epithelial cell (HEEC-1)were kindly provided by Dr. Y. Shimada (Kyoto University, Kyoto, Japan). The KYSE cell lines were cultured in RPMI 1640 medium (Life Technologies, Inc., Grand Island, NY) containing 10% heat-inactivated fetal bovine serum (FBS; BioWhittaker, Verviers, Belgium) in a humidified atmosphere of 5% CO2 and maintained in continuous exponential growth by passage every 3 days. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1530

40 Samples

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(Submitter supplied) A normal neonatal human diploid fibroblast strain MJ90 was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Sigma-Aldrich Japan) supplemented with 2 mM L-glutamine (Life Technologies) and 10% fetal calf serum (FCS; Life Technologies). A normal human umbilical vein endothelial cell strain HUE142-2 were grown in MCDB151 medium (Sigma-Aldrich Japan) supplemented with 15% fetal bovine serum, 5 µg/ml of heparin (Sigma), and 5 ng/ml of recombinant acidic fibroblast growth factor in a plastic flask (Corning) precoated with bovine fibronectin solution (1 µg/cm2, Wako).The 40 µg of plasmid hTRT/FLAG or hTERTn2 (from Prof. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1760

Platform:

GPL1530

12 Samples

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Analysis of normal fibroblasts and endothelial cells, at various population doubling levels (PDLs), following human telomerase reverse transcriptase (hTERT) transfection. hTERT mediates cellular immortalization. Results identify cell-type specific transcriptional responses during immortalization.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 2 cell type, 5 time sets

Platform:

GPL1530

Series:

GSE1900

12 Samples

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(Submitter supplied) This array was made in the laboratory of Dr. Yasushi Okazaki at the Riken Genome Science Center, Yokohama Institute in Japan. The array contains 21,168 spots including 20784 spots of human cDNA clones purchased from ResGen™ (Invitrogen Corporation, Carlsbad, CA, USA) and the remaining spots are the internal and exogenous controls spotted on every subgrid as positive and negative controls. Keywords = RIKEN human 21K cDNA

Organism:

Homo sapiens

1 DataSet

2 Series

52 Samples

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