GEO DataSets

(Submitter supplied) Non-coding RNA regulatory elements are important for viral replication, making them promising targets for therapeutic intervention. However, regulatory RNA is challenging to detect and characterise using classical structure-function assays. Here, we present in cell Mutational Interference Mapping Experiment (in cell MIME) as a way to define RNA regulatory landscapes at single nucleotide resolution under native conditions. more...

Organism:

Human immunodeficiency virus 1

Type:

Other

Platform:

GPL20319

27 Samples

Download data: TXT

(Submitter supplied) Vertebrate genomes exhibit marked CG-suppression, that is lower than expected numbers of 5’-CG-3’ dinucleotides1. This feature is likely due to C-to-T mutations that have accumulated over hundreds of millions of years, driven by CG-specific DNA methyl transferases and spontaneous methyl-cytosine deamination. Remarkably, many RNA viruses of vertebrates that are not substrates for DNA methyl transferases mimic the CG-suppression of their hosts2-4. more...

Organism:

Human immunodeficiency virus 1

Type:

Other

Platform:

GPL20319

2 Samples

Download data: XLS

(Submitter supplied) Integrase CLIP-seq experiments were conducted on wild-type and eccentric HIV-1 virions generated in the presence of allosteric integrase inhibitors and IN K264/266A and R269/K273A mutations

Organism:

Human immunodeficiency virus 1

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL22068 GPL20319

5 Samples

Download data: TXT

(Submitter supplied) During HIV-1 assembly, the structural protein Pr55Gag specifically selects viral genomic RNA by interacting with packaging signals present in the 5’ untranslated region (UTR). Despite a large number of studies, Pr55Gag binding to the genomic RNA remains poorly understood. To precisely define the Pr55Gag binding site we performed Mutational Interference Mapping Experiment on the 5’UTR and the beginning of the gag gene. more...

Organism:

Human immunodeficiency virus 1

Type:

Other

Platform:

GPL20319

18 Samples

Download data: TXT