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Items: 6

1.

scSLAM-seq reveals core features of transcription dynamics in single cells

(Submitter supplied) Single cell RNA sequencing so far only depicts cellular transcriptomes at a single time point with low temporal resolution for kinetic changes. Here, we present a single-cell approach based on metabolic RNA labelling for combined sequencing of total and newly transcribed RNA as well as computational analysis of the RNA present prior to labelling. We apply it to cytomegalovirus infection of fibroblasts and reveal its potential for delineating alterations in transcriptional activity and decay in single cells under perturbed experimental conditions.
Organism:
Homo sapiens; Mus musculus; Murid betaherpesvirus 1
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL25867 GPL19057 GPL25526
124 Samples
Download data: H5, TSV, TXT
Series
Accession:
GSE115612
ID:
200115612
2.

Illumina NextSeq 500 (Murid betaherpesvirus 1)

Organism:
Murid betaherpesvirus 1
1 Series
4 Samples
Download data
Platform
Accession:
GPL25867
ID:
100025867
3.

viral.D

Organism:
Murid betaherpesvirus 1
Source name:
mcmv viral stock
Platform:
GPL25867
Series:
GSE115612
Download data
Sample
Accession:
GSM3496510
ID:
303496510
4.

viral.C

Organism:
Murid betaherpesvirus 1
Source name:
mcmv viral stock
Platform:
GPL25867
Series:
GSE115612
Download data
Sample
Accession:
GSM3496509
ID:
303496509
5.

viral.B

Organism:
Murid betaherpesvirus 1
Source name:
mcmv viral stock
Platform:
GPL25867
Series:
GSE115612
Download data
Sample
Accession:
GSM3496508
ID:
303496508
6.

viral.A

Organism:
Murid betaherpesvirus 1
Source name:
mcmv viral stock
Platform:
GPL25867
Series:
GSE115612
Download data
Sample
Accession:
GSM3496507
ID:
303496507
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Supplemental Content

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