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Items: 6

1.

Zinc deficiency impacts CO2 assimilation and disrupts copper homeostasis in Chlamydomonas reinhardtii

(Submitter supplied) Zinc is an essential nutrient because of its role in catalysis and in stabilizing protein structure, but excess zinc can also be deleterious. Four nutritional zinc states have been identified in the alga Chlamydomonas reinhardtii: zinc toxic, zinc replete, zinc deficient and zinc limited. Growth is inhibited in zinc-limited and zinc toxic cells relative to zinc-replete cells, while zinc-deficiency is visually asymptomatic but distinguished by the accumulation of transcripts encoding ZIP family transporters. more...
Organism:
Chlamydomonas reinhardtii
Type:
Expression profiling by high throughput sequencing
Platform:
GPL9152
1 Sample
Download data: TXT
Series
Accession:
GSE41096
ID:
200041096
2.

Small RNA profiling of strains and lifecycle of Chlamydomonas reinhardtii

(Submitter supplied) High throughput sequencing was used to investigate the production of small RNAs from in Chlamydomonas in different strains and different stages of the life cycle. The association between these and methylation was assessed using genomic sequencing, comparing a sequenced genome with one enriched for methylated DNA sequence by immuno-precipitation.
Organism:
Chlamydomonas reinhardtii
Type:
Non-coding RNA profiling by high throughput sequencing; Methylation profiling by high throughput sequencing
Platform:
GPL9152
14 Samples
Download data: TXT
Series
Accession:
GSE23379
ID:
200023379
3.

SINGLET OXYGEN RESISTANT 1 links reactive electrophile signaling to singlet oxygen acclimation in Chlamydomonas reinhardtii

(Submitter supplied) To identify components involved in the signal transduction and activation of the singlet oxygen-mediated response, a mutant selection was performed. This led to the isolation of the singlet oxygen resistant 1 (sor1) mutant, which is more tolerant to singlet oxygen-producing chemicals and shows a constitutively higher expression of GPXH and GSTS1. Map-based cloning revealed that the SOR1 gene encodes a novel bZIP transcription factor, which seems to control its own expression as well as that of a large number of oxidative stress response and detoxification genes. more...
Organism:
Chlamydomonas reinhardtii
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL9152 GPL9100
2 Samples
Download data: TXT
Series
Accession:
GSE33548
ID:
200033548
4.

A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii

(Submitter supplied) Interest in exploiting algae as a biofuel source and the role of nutrient deficiency in inducing triacylglyceride (TAG) accumulation in cells necessitates a strategy to efficiently formulate species-specific culture media that can be easily manipulated. Using the reference organism Chlamydomonas reinhardtii, we tested the hypothesis that modeling trace element supplements on the ionomes of cells would result in optimized cell growth. more...
Organism:
Chlamydomonas reinhardtii
Type:
Expression profiling by high throughput sequencing
Platform:
GPL9152
2 Samples
Download data: TXT
Series
Accession:
GSE25622
ID:
200025622
5.

Systems analysis of Chlamydomonas Cu nutrition reveals connections between Cu and multiple O2-dependent metabolic steps

(Submitter supplied) We used digital gene expression (NlaIII sequence tags) and RNA-Seq to compare the transcriptomes of Cu-replete vs. Cu–deficient Chlamydomonas wild-type cells to reveal dozens of mRNAs whose abundance is modified. Half of the corresponding genes are targets of CRR1, a master regulator of nutritional copper sensing, and are associated with candidate CRR1 binding sites. The targets include many plastid-localized proteins, like FDX5 encoding a ferredoxin isoform, and CGL78, encoding a protein conserved in the green lineage, indicative of modified plastid metabolism. more...
Organism:
Chlamydomonas reinhardtii
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL9152 GPL9100
34 Samples
Download data: TXT, WIG
Series
Accession:
GSE25124
ID:
200025124
6.

RNA-seq analysis of the transcriptome from Sulfur Deprivation Chlamydomonas cells

(Submitter supplied) The Chlamydomonas reinhardtii transcriptome was characterized from nutrient-replete and sulfur-depleted wild-type and snrk2.1 mutant cells; the mutant is null for the regulatory serine-threonine kinase SNRK2.1, which is required for acclimation to sulfur deprivation. The transcriptome analyses involved microarray hybridization and RNA-seq technology; RT-qPCR evaluation of the data obtained by these techniques showed that RNA-seq is significantly more quantitative than microarray hybridizations. more...
Organism:
Chlamydomonas reinhardtii
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL9152 GPL9100
4 Samples
Download data: TXT, XLS
Series
Accession:
GSE17970
ID:
200017970
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Supplemental Content

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