GEO DataSets

(Submitter supplied) Sugarcane stalk borer larvae were grown on artificial diet and maintained at 25°C and 60±10% relative humidity with a 14 h/10 h light/dark cycle. Second instar larvae were maintained under fasting conditions for 18 h and transferred to two-month old plants (genotype SP80-3280, CTC, Brazil). Leaves were collected after 30 min and 24 h of exposure to herbivory for the control and experimental groups. Two plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of stress response

Organism:

Saccharum officinarum; Saccharum hybrid cultivar SP80-3280

Type:

Expression profiling by array

Platform:

GPL3799

8 Samples

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(Submitter supplied) Six 384-well plates representing 1830 unique SUCAST SAS were rearrayed for spotting Protocol: Sugarcane cDNA plasmid clones obtained from the SUCEST collection were re-arranged and amplified in 100ul PCR reactions (40 cycles, annealing at 51°C), directly from bacterial clones in culture, using T7 and SP6 primers. PCR products were purified by filtration using 96 well filter plates (Millipore Multiscreen MAFBN0B50). more...

Organism:

Saccharum officinarum

9 Series

124 Samples

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