GEO DataSets

(Submitter supplied) Chick pinealocytes exhibit all the characteristics of a complete circadian system, comprising photoreceptive inputs, molecular clockworks and an easily measured rhythmic output, melatonin biosynthesis. We used microarray analysis to investigate the expression of approximately 8000 genes within cultured pinealocytes subjected to both LD and DD cycles. We report that a reduced subset of genes were rhythmically expressed in vitro compared to those previously published in vivo, and that gene expression rhythms were lower in amplitude, although the functional distribution of the rhythmic transcriptome was largely similar. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3731

196 Samples

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(Submitter supplied) The cDNA microarrays were prepared based upon approximately 4,000 PCR products from each of the two chicken pineal cDNA libraries constructed, for a total of ~8,000 ESTs. Briefly, the cDNA in plasmids from each library were PCR amplified using flanking primers (SK and T7), purified by ethanol precipitation using ammonium acetate, and placed in the wells of 96 well plates at a concentration of 50 ug/ml in 3X SSC. more...

Organism:

Gallus gallus

1 Series

196 Samples

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