GEO DataSets

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Ovis aries

Type:

Expression profiling by array

Platforms:

GPL4072 GPL4077

25 Samples

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(Submitter supplied) Afferent Lymph was collected from up to 5 pen raised, nematode naïve, outbreed Romney sheep (Tag No’s 1001, 1002, 1003, 1004, 1006) over a 12 week period. During this time a Pre-Infection sample was collected, the sheep were immunised by 3 truncated (2 week) T. colubiformis infections (40,000 Tc) followed by a Challenge Infection with 40,000 T. colubiformis L3 (Challenge Infection weeks 1-3). The Challenge Infection will enable us to determine changes in gene expression when the sheep gut rejects the parasitic nematodes.

Organism:

Ovis aries

Type:

Expression profiling by array

Platforms:

GPL4077 GPL4072

12 Samples

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(Submitter supplied) This array contains spotted PCR products from an ovine EST collection. Included are housekeeping genes, water blanks (negative PCR) and no DNA blanks (to estimate carryover) Protocol: Ovine EST inserts were amplified in 96 well plates using the universal primers SP6 and T7 in 50 ul reactions (1.5 mM MgCl2, 2 mM each dNTP, 45 pmol primers and 2.5 units AB Red Hot Taq polymerase). The reactions were denatured at 94C for 3 minutes then cycled 36 times at 94C for 45 sec, 55C for 45 sec and 72C for 60 sec. more...

Organism:

Ovis aries

3 Series

20 Samples

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