GSM6076923[Accession] - GEO DataSets

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Items: 4

Select item 2002018741.

Timing of Germ Layer Initiation Linked to Spatially Patterned Onset of Genome Activation

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Xenopus laevis

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21248
106 Samples

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Accession:: GSE201874

ID:: 200201874

PubMed Full text in PMC Similar studies

Select item 2002018332.

Nascent Transcriptome of AP and VP from Xenopus laevis Embryos at 5-9 hpf (stage 7-9)

(Submitter supplied) To characterize the spatial patterns of zygtoic transcription during zygotic genome activation (ZGA) of Xenopus laevis embryos, we microinjected 5-ethynyl uridine (EU) into 1-cell stage embryos and isolated total RNAs from whole embryos at 5, 6, 7, 8 and 9 hours post-fertilization (hpf) at room temperature, respectively, covering the stages of pre-ZGA to widespread ZGA (stage 7-9). The animal pole (AP, ~ 1/3 at the top region of embryo) and the vegetal pole (VP,~ 1/3 at the bottom region of embryo) regions were dissected and used for total RNA isolation. more...