GEO DataSets

(Submitter supplied) In vitro production of cattle embryos over the past two decades has revealed several negative impacts, which have been attributed to the artificial microenvironment. Studies of embryos produced in vitro clearly point to aberrant gene expression levels. So far, the causal association between phenotype and measured gene expression has not led to substantial improvement of in vitro production systems. The aim of the present study was to generate a unique dataset composed of microarray-derived relative transcript abundance values for blastocysts produced in ten in vitro systems differing primarily in culture medium formulation. Between-group comparisons allowed us to determine the level of overall similarity between systems relative to in vivo reference embryos. The use of the dataset to contrast all in vitro treatments with the in vivo blastocysts pointed to a single common gene network. The “boutique” array contained a panel of novel uncharacterized transcripts that were expressed variably depending on the medium in which the blastocysts were produced. These novel transcripts were expressed differentially in blastocysts even as carry-over from conditions encountered seven days earlier during oocyte maturation. All of the selected novel candidates that were thus expressed were from intergenic regions. The function of this long non-coding RNA remains unknown but points clearly to an additional level of complexity in early embryo development.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13261

65 Samples

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(Submitter supplied) Templates of a set of candidates isolated from a collection of 4 subtracted cDNA libraries were amplified by PCR. The amplicons (of 450 bp on average) were purified to remove excess primers and free nuleotides and the remaining DNA was used as the source of DNA to fabricate the microarray. DNA was diluted in DMSO to increase surface tension and deposition was performed using a Vertel arrayer (now VerArray from BioRad) using solid pins. more...

Organism:

Bos taurus

1 Series

65 Samples

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