GSM76479[Accession] - GEO DataSets

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Items: 3

Select item 2000033991.

Identification of BABY BOOM downstream targets

(Submitter supplied) A glucocorticoid-regulated BBM protein (35S:BBM-GR) was used in combination with microarray analysis to identify genes directly activated by BBM. We employed the system described by (Lloyd et al., 1994) in which dexamethasone (DEX) and cycloheximide (CHX) are applied together to respectively, induce nuclear localization of the BBM-GR protein and prevent translation of the primary targets mRNAs. In this way it is possible to identify direct targets of a transcriptional activator by comparing gene expression profiles between DEX+CHX-treated transgenic and wild-type tissues. more...

Organism:: Arabidopsis thaliana

Type:: Expression profiling by array

Platform:: GPL2907
10 Samples

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Accession:: GSE3399

ID:: 200003399

Select item 1000029072.

Qiagen-Operon Arabidopsis 28.8k Genome Array (AtOligo 1.16.2.Y)

(Submitter supplied) ArrayIt® brand SuperAmine 2 Microarray Substrates (25 x 76 mm). 48 pins=12 metarows and 4 metacolumns, and the subarrays comprise 24 columns and 25 rows (20 x 60 mm). Protocol: (1) Microarray production and probe immobilization: http://ag.arizona.edu/microarray/protocol1A.doc (2) Oligo/gene list: http://omad.operon.com/download/storage/arabidopsis_V1.0.2_genelist_s+.xls.zip (3) Oligo data sheet: http://omad.operon.com/download/storage/arabidopsis_V1.0.1_datasheet.pdf