GEO DataSets

(Submitter supplied) New experiences can trigger changes in gene expression in the brain. To understand this phenomenon better, we studied zebra finches hearing playbacks of birdsong. Earlier research had shown that initial playbacks of a novel song transiently increase the ZENK (ZIF-268, EGR1, NGFIA, KROX-24) mRNA in the auditory forebrain, but the response selectively habituates after repetition of the stimulus. Here, using DNA microarray analysis, we show that novel song exposure induces rapid changes in thousands of RNAs, with even more RNAs decreasing than increasing. more...

Organism:

Taeniopygia guttata

Type:

Expression profiling by array

Platform:

GPL9554

18 Samples

Download data: GPR

(Submitter supplied) cDNAs are arrayed in Genetix 384 well plates and stored frozen. Plates are thawed and spun down. Plates are placed in storage hotel of an Omnigrid 100 (Genomic Solutions) Slides are printed in batches of 100 using 1.5M Betaine + 3 x SSC Following printing slides are baked at 80C for 2 hours Slides are washed in 0.2% SDS followed by three water washes and an ethanol wash Slides are dried by spinning in a centriguge at 1000 RPM for 3 minutes Slides are stored in orange Corning boxes 25 per box Storage boxes are purged with argon and sealed under vacuum

Organism:

Taeniopygia guttata

22 Series

692 Samples

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