GEO DataSets

(Submitter supplied) The oleaginous red yeast species Rhodotorula toruloides, a prominent environmental basidiomycetous yeast, has garnered significant interest for its remarkable capacity to utilize main carbon sources present in lignocellulosic hydrolysates, such as glucose, xylose, and acetic acid, and to efficiently produce lipids and carotenoids. This species can also efficiently use other less usual and difficult-to-catabolize C-sources, as is the case of the acid sugar D-galacturonic acid and the neutral sugar L-arabinose, present in hydrolysates from pectin-rich agro-industrial residues. more...

Organism:

Rhodotorula toruloides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32811

4 Samples

Download data: TXT

(Submitter supplied) Insufficient biosynthesis efficiency can be a major obstacle to engineer oleaginous yeasts to overproduce very long-chain fatty acids (VLCFAs) during the lipogenic phase. Taking nervonic acid (NA, C24:1) as an example, we overcame the bottleneck to overproduce NA in engineered Rhodosporidium toruloides by improving the biosynthesis of VLCFAs during the lipogenic phase. First, evaluating the catalytic preferences of three plant-derived ketoacyl-CoA synthases rationally guided reconstructing efficient NA biosynthetic pathway in R. more...

Organism:

Rhodotorula toruloides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32811

18 Samples

Download data: TXT

(Submitter supplied) Oleaginous yeasts are capable of accumulating high levels of intracellular storage lipids from excess carbon during times when other key nutrients are limited. The basidiomycete yeast Rhodosporidium toruloides is an emerging host for microbial cell factory applications thanks to its naturally high lipid and carotenoid production. However, the engineering toolbox in this non-model host is limited and is currently a bottleneck for implementation of metabolic engineering strategies. more...

Organism:

Rhodotorula toruloides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32811

12 Samples

Download data: TSV

(Submitter supplied) Transcriptome sequencing to understand the impact of integration copy number and genome location on heterologous gene expression in R. toruloides

Organism:

Rhodotorula toruloides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29003

36 Samples

Download data: TXT

(Submitter supplied) Background: A key prerequisite for pathway engineering is the development of genetic tools and resources. Rhodosporidium toruloides is emerging as a promising host for the production of bioproducts from lignocellulosic biomass. However, there is a lack of characterized promoters to drive expression of heterologous genes for strain engineering in R. toruloides. Results: The resulting data describes a set of native R. more...

Organism:

Rhodotorula toruloides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26239

10 Samples

Download data: TAB

(Submitter supplied) Biorefining of renewable feedstocks is one of the most promising routes to replace fossil-based products. Since many common fermentation hosts, such as Saccharomyces cerevisiae, are naturally unable to convert many component plant cell wall polysaccharides, the identification of organisms with broad catabolism capabilities represents an opportunity to expand the range of substrates used in fermentation biorefinery approaches. more...

Organism:

Rhodotorula toruloides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26239

9 Samples

Download data: TAB

(Submitter supplied) Lipid accumulation by oleaginous microorganisms is of great scientific interest and biotechnological potential. While nitrogen limitation has been routinely employed, low-cost raw materials usually contain rich nitrogenous components, thus preventing from efficient lipid production. Inorganic phosphate (Pi) limitation has been found sufficient to promote conversion of sugars into lipids, yet the molecular basis of cellular response to Pi-limitation and concurrent lipid accumulation remains elusive. more...

Organism:

Rhodotorula toruloides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24890

2 Samples

Download data: TXT

(Submitter supplied) The goal of the study is to use Next generation sequencing (RNA-seq) to study the underlying regulation of glycerol metabolism in mixed culture fermentation (glucose and glycerol) of Rhodosporidium toruloides. We sequenced the RNA from 4 different samples in the mixed culture (glucose and glycerol) with 2 replicates each. Transcriptional profiles showed that glycerol might be produced intracellularly and glycerol kinase (GUT1) and glycerol 3–phosphate dehydrogenase (GUT2) enzymes were not down-regulated in the presence of glucose at the transcriptional level. more...

Organism:

Rhodotorula toruloides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21062

8 Samples

Download data: TXT

(Submitter supplied) Nitrogen limitation is a major regulator to initiate lipid overproduction in oleaginous fungi. To examine the influence of nitrogen starvation, chemiostat cultures of R. toruloides in defined media with abundant ammonium (MM) or minute ammonium (MM-N) were performed to obtain steady-state samples. Then Illumina's digital gene expression (DGE) technology was used for high-throughput transcriptome profiling of these samples.

Organism:

Rhodotorula toruloides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15759

2 Samples

Download data: TXT