GEO DataSets

(Submitter supplied) mRNA gene expression was measured in intact female Sprague-Dawley rats at 6 (young), 26 (adult) and 52 (older) weeks of age at the time of fracture. Samples were collected at 0, 0.4, 1, 2, 4, and 6 weeks after fracture. RNA from two rats were pooled for each Affymetrix Rat U34A array. Mid-shaft, simple, transverse left femoral fractures were induced after retrograde intramedullary rod fixation with a Bonnarens and Einhorn device. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS509

Platform:

GPL85

54 Samples

Download data: CEL

(Submitter supplied) A study of rat femoral fracture healing in young (6 weeks old at fracture), adult (26 weeks old at fracture), and old (52 weeks old at fracture) rats. Samples were collected at time of surgery (intact controls) and at 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks after fracture. Samples were the mid third of the femoral length including the external callus, cortical bone and marrow elements. Fracture was stabilized with an intramedullary rod prior to fracture with a Bonnarens and Einhorn device. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS1995

Platform:

GPL85

18 Samples

Download data: CEL

(Submitter supplied) Study of rat femur fracture healing in young (6 weeks old), adult (26 weeks old), and older (52 weeks old) rats with samples collected at 0 time (no fracture) and at 0.4, 1, 2, 4, and 6 weeks after fracture. RNA from two rats were pooled for each array. Keywords = rat, femur, fracture, age, time Keywords: time-course

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL85

18 Samples

Download data: CEL

(Submitter supplied) mRNA gene expression was measured in rats at 6 (young), 26 (adult) and 52 (older) weeks of age at the time of fracture. Samples were collected at 0, 0.4, 1, 2, 4, and 6 weeks after fracture. RNA from two rats were pooled for each Affymetrix Rat U34A array. Keywords = rat Keywords = fracture Keywords = age Keywords = time Keywords = femur Keywords: time-course

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL85

18 Samples

Download data: CEL

Analysis of healing femurs of 6, 26, and 52-week-old Sprague-Dawley females at various time points up to 6 weeks after fracture. 6, 26, and 52-week-old animals regain normal skeletal biomechanics 4 weeks, 10 weeks, and 6 months after fracture, respectively.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 3 age, 2 protocol, 6 time sets

Platform:

GPL85

Series:

GSE1371

18 Samples

Download data: CEL

Temporal analysis of gene expression following femoral fracture. Female Sprague-Dawleys at 6 (young), 26 (adult) and 52 (older) weeks of age at time of fracture. Samples analyzed at 0, 3 days, 1, 2, 4, and 6 weeks after fracture.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 3 age, 6 time sets

Platform:

GPL85

Series:

GSE594

36 Samples

Download data: CEL

(Submitter supplied) Mid-shaft fracture stimulates bone lengthening by increasing linear growth at the growthplate. This project studied changes in mRNA in the proximal growthplate after a mid-shaft fracture in a rat model. Keywords: time course, fracture, growthplate, rat

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS2020

Platform:

GPL1355

16 Samples

Download data: CEL, TXT, XLS

Analysis of proximal femoral growth plate at various time points up to 6 weeks following midshaft fracture in 4 week-old Sprague-Dawley females. Growth plates from fractured femora and intact contralateral femora compared. Midshaft femoral fracture stimulates linear growth of the bone.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 other, 2 shock, 8 time sets

Platform:

GPL1355

Series:

GSE3298

16 Samples

Download data: CEL

(Submitter supplied) This is a study of femoral fracture healing in female rats 16 weeks old at fracture to compare intramedullary nailing, screw and plate fixation, and sham surgery. The sham surgery group received a surgical exposure of the femur, but no fracture, no plate, and no nail. Samples were collected at 1 day, 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks after surgery. Each sample is a pool of RNA from three rats from the same surgery group at the same time point after fracture. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS1853

Platform:

GPL85

19 Samples

Download data

Comparison of healing diaphyseal femoral fractures treated with intramedullary nail fixation or with internal plate fixation. Expression profiles examined at various time points up to 6 weeks post-treatment. Results provide insight into the effect of fracture fixation technique on fracture repair.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 3 protocol, 3 shock, 7 time sets

Platform:

GPL85

Series:

GSE1685

19 Samples

Download data

(Submitter supplied) Sprague-Dawley rats were placed on an ethanol-containing or pair-fed Lieber and DeCarli diets for 4 wks prior to surgical fracture. Following insertion of a medullary pin, a closed mid-diaphyseal fracture was induced using a Bonnarens and Einhorn fracture device. At 3 days post-fracture, the region of the fracture calluses were harvested from the right hind-limb. RNA was extracted and microarray analysis was conducted against the entire rat genome to study the effects of alcohol-consumption on the fracture healing.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL7471

6 Samples

Download data: GPR

(Submitter supplied) Profiling of miRNA expressions comparing standard fracture healing models with nonunion models in rats

Organism:

Rattus norvegicus; Human alphaherpesvirus 2; Merkel cell polyomavirus; Homo sapiens; Murid gammaherpesvirus 4; Betapolyomavirus hominis; Mus musculus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; Human immunodeficiency virus 1; human gammaherpesvirus 4; JC polyomavirus; Human gammaherpesvirus 8; Mus musculus cytomegalovirus 2; Betapolyomavirus macacae

Type:

Non-coding RNA profiling by array

Platform:

GPL11434

2 Samples

Download data: TXT

(Submitter supplied) mRNA used for the analysis of these microarrays were previously analyzed for 34 genes by reverse transcription - polymerase chain reaction in Desai BJ et al., J.Orthop.Trauma 17: 689-698, 2003. These two data sets were subsequently studied to compare the results from these two different methods for mRNA quantitation. The comparison was publised in "Comparison of mRNA gene expression by RT-PCR and DNA microarray" by W. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL85

10 Samples

Download data: CEL

(Submitter supplied) The phases of fracture healing have been well characterized. However, the exact source and genetic profile of the skeletal progenitors that participate in bone repair is somewhat unclear. Sox9 expression in skeletal elements precedes bone and cartilage formation and a Sox9+ cell type is retained in the adult periosteum. We hypothesized that Sox9+ periosteal cells are multipotent skeletal progenitors normally participating in fracture repair. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

4 Samples

Download data: TXT

(Submitter supplied) Vitamin A is the only known compound that produces spontaneous fractures in rats. In an effort to resolve the molecular mechanism behind this effect, we fed young rats high doses of vitamin A and performed a global transcriptional analysis of diaphyseal bone after one week, i.e. just before the first fractures appeared. Microarray gene expression analysis revealed that 68 transcripts were differentially expressed in hypervitaminotic cortical bone and 118 transcripts were found when the bone marrow was also included. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

16 Samples

Download data: CEL

(Submitter supplied) Phosphate is essential for healthy bone growth and plays an essential role in fracture repair. Although phosphate deficiency has been shown to impair fracture healing, the mechanisms involved in impaired healing are unknown. More recently, studies have shown that the effect of phosphate deficiency on the repair process varied based on the genetic strain of mice, which is not characterized. We used data from microarrays to (1) determine the effects of phosphate restriction on the biologic functions identified from the gene expression in fracture calluses; and (2) examine whether there are genetic differences within the primary biologic functions.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19485

239 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL11154

78 Samples

Download data

(Submitter supplied) Despite the advances in bone fracture treatment, a significant fraction of fracture patients will develop non-union. Most non-unions are treated with surgery since identifying the molecular causes of these defects is exceptionally challenging. In this study, compared with marrow bone, we generated a transcriptional atlas of human osteoprogenitor cells derived from healing callus and non-union fractures. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

31 Samples

Download data: TSV

(Submitter supplied) Despite the advances in bone fracture treatment, a significant fraction of fracture patients will develop non-union. Most non-unions are treated with surgery since identifying the molecular causes of these defects is exceptionally challenging. In this study, compared with marrow bone, we generated a transcriptional atlas of human osteoprogenitor cells derived from healing callus and non-union fractures. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL11154 GPL24676

47 Samples

Download data: TSV

(Submitter supplied) The long-term healing outcome of bone regeneration is known to depend not only on mechanical stability, but also on age and, more importantly, on the overlap of both parameters. Although especially the early healing period determines the healing outcome, it so far remains unclear which factor - mechanical loading or age - dominates this phase and how these factors interact on the molecular level in the early fracture hematoma. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

12 Samples

Download data: CEL